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Literature summary extracted from

  • Chang, W.; Guo, Y.; Wang, C.; Butch, S.; Rosenzweig, A.; Boal, A.; Krebs, C.; Bollinger Jr., J.
    Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics (2014), Science, 343, 1140-1144 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.20.3 structure of CarC/Fe(II)/2-oxoglutarate complex Pectobacterium carotovorum subsp. carotovorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.3 Fe2+ Fe(II)-dependent oxygenase. An Fe(IV)-oxo intermediate abstracts the hydrogen (H*) from C5, and tyrosine 165, a residue not visualized in the published structures of CarC lacking bound substrate, donates H* to the opposite face of the resultant radical. The reaction oxidizes the Fe(II) cofactor to Fe(III), limiting wild-type CarC to one turnover, but substitution of the H-donating tyrosine disables stereoinversion and confers to CarC the capacity for catalytic substrate oxidation Pectobacterium carotovorum subsp. carotovorum

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.3 Pectobacterium carotovorum subsp. carotovorum Q9XB59
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Synonyms

EC Number Synonyms Comment Organism
1.14.20.3 CarC
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Pectobacterium carotovorum subsp. carotovorum