Literature summary extracted from
Chang, W.; Guo, Y.; Wang, C.; Butch, S.; Rosenzweig, A.; Boal, A.; Krebs, C.; Bollinger Jr., J.
Mechanism of the C5 stereoinversion reaction in the biosynthesis of carbapenem antibiotics (2014), Science, 343, 1140-1144 .
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.14.20.3 |
structure of CarC/Fe(II)/2-oxoglutarate complex |
Pectobacterium carotovorum subsp. carotovorum |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.20.3 |
Fe2+ |
Fe(II)-dependent oxygenase. An Fe(IV)-oxo intermediate abstracts the hydrogen (H*) from C5, and tyrosine 165, a residue not visualized in the published structures of CarC lacking bound substrate, donates H* to the opposite face of the resultant radical. The reaction oxidizes the Fe(II) cofactor to Fe(III), limiting wild-type CarC to one turnover, but substitution of the H-donating tyrosine disables stereoinversion and confers to CarC the capacity for catalytic substrate oxidation |
Pectobacterium carotovorum subsp. carotovorum |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.20.3 |
Pectobacterium carotovorum subsp. carotovorum |
Q9XB59 |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.20.3 |
CarC |
- |
Pectobacterium carotovorum subsp. carotovorum |