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Literature summary extracted from

  • Liang, M.H.; Qv, X.Y.; Jin, H.H.; Jiang, J.G.
    Characterization and expression of AMP-forming acetyl-CoA synthetase from Dunaliella tertiolecta and its response to nitrogen starvation stress (2016), Sci. Rep., 6, 23445 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.1 expressed in Escherichia coli BL21(DE3) cells Dunaliella tertiolecta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 3.59
-
 potassium acetate at pH 8.0 and 37°C Dunaliella tertiolecta
6.2.1.1 4.66
-
 Sodium acetate at pH 8.0 and 37°C Dunaliella tertiolecta

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.1 plasma membrane
-
 Dunaliella tertiolecta 5886
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.1 ATP + acetate + CoA Dunaliella tertiolecta
-
 AMP + diphosphate + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Dunaliella tertiolecta A0A144QFN1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1 HisTrap affinity column chromatography Dunaliella tertiolecta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.1 0.885
-
 crude extract, at pH 8.0 and 37°C Dunaliella tertiolecta
6.2.1.1 52.873
-
 after 59.7fold purification, at pH 8.0 and 37°C Dunaliella tertiolecta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ATP + acetate + CoA
-
 Dunaliella tertiolecta AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + potassium acetate + CoA
-
 Dunaliella tertiolecta AMP + acetyl-CoA + potassium diphosphate
-
 ?
6.2.1.1 ATP + sodium acetate + CoA
-
 Dunaliella tertiolecta AMP + acetyl-CoA + sodium diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 ? x * 100000, thiordeoxin-tagged enzyme, SDS-PAGE Dunaliella tertiolecta
6.2.1.1 ? x * 79720, calculated from amino acid sequence Dunaliella tertiolecta

Synonyms

EC Number Synonyms Comment Organism
6.2.1.1 ACS
-
 Dunaliella tertiolecta
6.2.1.1 AMP-forming acetyl-CoA synthetase
-
 Dunaliella tertiolecta
6.2.1.1 AMP-forming ACS
-
 Dunaliella tertiolecta

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.1 37
-
-
 Dunaliella tertiolecta

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.1 8
-
-
 Dunaliella tertiolecta

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.1 ATP
-
 Dunaliella tertiolecta

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.1 Dunaliella tertiolecta isoelectric focusing
-
 6.7