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Literature summary extracted from

  • Hori, K.; Okano, S.; Sato, F.
    Efficient microbial production of stylopine using a Pichia pastoris expression system (2016), Sci. Rep., 6, 22201 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.19.64 pharmacology a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells Eschscholzia californica
1.14.19.64 synthesis a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells Eschscholzia californica
1.14.19.65 pharmacology a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells Eschscholzia californica
1.14.19.65 synthesis a microbial system is established for producing a protoberberine-type alkaloid (stylopine) in Pichia cells Eschscholzia californica

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.64 coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline) Eschscholzia californica
1.14.19.65 coexpression of cheilanthifoline synthase (CYP719A5), and stylopine synthase (CYP719A2) in Pichia pastoris. Biosynthetic enzyme expression is examined in a consolidated system with all genes expressed in one cell and a coculture system with three cell lines that each express a single gene were examined. Although both systems efficiently converted reticuline to stylopine, the consolidated system is more rapid and efficient than the co-culture system. However, substrate-feeding experiments reveal a decrease in the conversion efficiency in the consolidated system during successive cultures, whereas the conversion efficiency in the co-culture system remains constant. Thus, the final amount of stylopine produced from reticuline after successive feedings in the co-culture system is more than 150 nmoles from 750 nmoles of (R,S)-reticuline (375 nmoles of (S)-reticuline) Eschscholzia californica
1.21.3.3 expressed in Pichia pastoris strain GS115 Eschscholzia californica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.3.3 (S)-reticuline + O2 Eschscholzia californica
-
 (S)-scoulerine + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.64 Eschscholzia californica Q50LH3
-
-
1.14.19.65 Eschscholzia californica B5UAQ8
-
-
1.21.3.3 Eschscholzia californica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.3.3 (S)-reticuline + O2
-
 Eschscholzia californica (S)-scoulerine + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.64 CYP719A2
-
 Eschscholzia californica
1.14.19.65 CYP719A5
-
 Eschscholzia californica
1.21.3.3 BBE
-
 Eschscholzia californica
1.21.3.3 berberine bridge enzyme
-
 Eschscholzia californica