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Literature summary extracted from

  • Phatarphekar, A.; Rokita, S.E.
    Functional analysis of iodotyrosine deiodinase from Drosophila melanogaster (2016), Protein Sci., 25, 2187-2195 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.1.1 expressed in Escherichia coli Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
1.21.1.1 E154Q the mutation diminishes the affinity of the enzyme for 3,5-diiodo-L-tyrosine by 30fold Drosophila melanogaster
1.21.1.1 K179Q the mutation diminishes the affinity of the enzyme for 3,5-diiodo-L-tyrosine by 46fold Drosophila melanogaster
1.21.1.1 Y158F the mutation results in a 7fold decrease in the enzyme's affinity for 3,5-diiodo-L-tyrosine Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.1.1 0.008
-
 3-bromo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
1.21.1.1 0.014
-
 3-iodo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
1.21.1.1 0.021
-
 3-chloro-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.1.1 3-iodo-L-tyrosine + NADP+ + iodide Drosophila melanogaster
-
 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 L-tyrosine + NADP+ + iodide Drosophila melanogaster
-
 3-iodo-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 L-tyrosine + NADP+ + iodide Drosophila melanogaster overall reaction 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.1.1 Drosophila melanogaster E1JIB2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.1.1 Ni2+ affinity column chromatography Drosophila melanogaster

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.1.1 3-bromo-L-tyrosine + NADP+ + bromide
-
 Drosophila melanogaster 3,5-dibromo-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 3-chloro-L-tyrosine + NADP+ + chloride
-
 Drosophila melanogaster 3,5-dichloro-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 3-iodo-L-tyrosine + NADP+ + iodide
-
 Drosophila melanogaster 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 L-tyrosine + NADP+ + iodide
-
 Drosophila melanogaster 3-iodo-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 L-tyrosine + NADP+ + iodide overall reaction Drosophila melanogaster 3,5-diiodo-L-tyrosine + NADPH + H+
-
 ?
1.21.1.1 additional information no activity with 3-fluoro-L-tyrosine Drosophila melanogaster ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.21.1.1 IYD
-
 Drosophila melanogaster

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.1.1 0.05
-
 3-chloro-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
1.21.1.1 0.069
-
 3-bromo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
1.21.1.1 0.12
-
 3-iodo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.21.1.1 FMN
-
 Drosophila melanogaster
1.21.1.1 NADP+
-
 Drosophila melanogaster

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.21.1.1 2400
-
 3-chloro-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
1.21.1.1 9000
-
 3-iodo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster
1.21.1.1 9000
-
 3-bromo-L-tyrosine at pH 7.4, temperature not specified in the publication Drosophila melanogaster