BRENDA - Enzyme Database

Heterologous expression, characterization and application of a new beta-xylosidase identified in solventogenic Clostridium sp. strain BOH3

Li, T.; Wu, Y.; He, J.; Process Biochem. 67, 99-104 (2018)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.37
SDS
118% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Tween 80
120% activity at 1 mM
Clostridium sp. BOH3
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
4-Aminobenzoic acid
about 90% residual activity at 10 mM
Clostridium sp. BOH3
3.2.1.37
Ag+
complete inhibition at 0.1 mM
Clostridium sp. BOH3
3.2.1.37
EDTA
about 45% residual activity at 10 mM
Clostridium sp. BOH3
3.2.1.37
Fe2+
about 70% residual activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Hg2+
complete inhibition at 0.1 mM
Clostridium sp. BOH3
3.2.1.37
Zn2+
about 75% residual activity at 1 mM
Clostridium sp. BOH3
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.37
2.71
-
4-nitrophenyl alpha-D-xylopyranoside
at pH 6.0 and 40°C
Clostridium sp. BOH3
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.37
Ca2+
148% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Co2+
139% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Mn2+
135% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
additional information
not influenced by Na+, K+ and Ni2+
Clostridium sp. BOH3
3.2.1.37
Zn2+
enzyme activity can be enhanced more than 1.63times by addition of 0.1 mM Zn2+
Clostridium sp. BOH3
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.2.1.37
Clostridium sp. BOH3
A0A024A6V9
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.2.1.37
4-nitrophenyl alpha-D-xylopyranoside + H2O
-
746353
Clostridium sp. BOH3
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
3.2.1.37
4-nitrophenyl alpha-L-arabinopyranoside + H2O
3.2% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
746353
Clostridium sp. BOH3
4-nitrophenol + alpha-L-arabinose
-
-
-
?
3.2.1.37
beechwood xylan + H2O
1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
birchwood xylan + H2O
1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
xylobiose + H2O
-
746353
Clostridium sp. BOH3
D-xylose
-
-
-
?
3.2.1.37
xylopentaose + H2O
-
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
xylotetraose + H2O
-
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
xylotriose + H2O
-
746353
Clostridium sp. BOH3
xylobiose + D-xylose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.37
?
x * 61000, calculated from amino acid sequence
Clostridium sp. BOH3
3.2.1.37
?
x * 80000, thioredoxin-tagged enzyme, SDS-PAGE
Clostridium sp. BOH3
Synonyms
EC Number
Synonyms
Commentary
Organism
3.2.1.37
beta-xylosidase
-
Clostridium sp. BOH3
3.2.1.37
Bxyl
-
Clostridium sp. BOH3
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
40
-
-
Clostridium sp. BOH3
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
50
-
the enzyme keeps almost 100% of its initial activity when incubating below 50°C for 2 h at pH 6.0
Clostridium sp. BOH3
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
5
6
-
Clostridium sp. BOH3
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.2.1.37
SDS
118% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Tween 80
120% activity at 1 mM
Clostridium sp. BOH3
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.37
4-Aminobenzoic acid
about 90% residual activity at 10 mM
Clostridium sp. BOH3
3.2.1.37
Ag+
complete inhibition at 0.1 mM
Clostridium sp. BOH3
3.2.1.37
EDTA
about 45% residual activity at 10 mM
Clostridium sp. BOH3
3.2.1.37
Fe2+
about 70% residual activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Hg2+
complete inhibition at 0.1 mM
Clostridium sp. BOH3
3.2.1.37
Zn2+
about 75% residual activity at 1 mM
Clostridium sp. BOH3
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.37
2.71
-
4-nitrophenyl alpha-D-xylopyranoside
at pH 6.0 and 40°C
Clostridium sp. BOH3
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.2.1.37
Ca2+
148% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Co2+
139% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
Mn2+
135% activity at 1 mM
Clostridium sp. BOH3
3.2.1.37
additional information
not influenced by Na+, K+ and Ni2+
Clostridium sp. BOH3
3.2.1.37
Zn2+
enzyme activity can be enhanced more than 1.63times by addition of 0.1 mM Zn2+
Clostridium sp. BOH3
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.2.1.37
4-nitrophenyl alpha-D-xylopyranoside + H2O
-
746353
Clostridium sp. BOH3
4-nitrophenol + beta-D-xylopyranose
-
-
-
?
3.2.1.37
4-nitrophenyl alpha-L-arabinopyranoside + H2O
3.2% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
746353
Clostridium sp. BOH3
4-nitrophenol + alpha-L-arabinose
-
-
-
?
3.2.1.37
beechwood xylan + H2O
1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
birchwood xylan + H2O
1.8% activity compared to 4-nitrophenyl alpha-D-xylopyranoside
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
xylobiose + H2O
-
746353
Clostridium sp. BOH3
D-xylose
-
-
-
?
3.2.1.37
xylopentaose + H2O
-
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
xylotetraose + H2O
-
746353
Clostridium sp. BOH3
?
-
-
-
?
3.2.1.37
xylotriose + H2O
-
746353
Clostridium sp. BOH3
xylobiose + D-xylose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.37
?
x * 61000, calculated from amino acid sequence
Clostridium sp. BOH3
3.2.1.37
?
x * 80000, thioredoxin-tagged enzyme, SDS-PAGE
Clostridium sp. BOH3
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.37
40
-
-
Clostridium sp. BOH3
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.37
50
-
the enzyme keeps almost 100% of its initial activity when incubating below 50°C for 2 h at pH 6.0
Clostridium sp. BOH3
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.37
5
6
-
Clostridium sp. BOH3