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Literature summary extracted from

  • Akram, F.; Haq, I.; Mukhtar, H.
    Gene cloning, characterization and thermodynamic analysis of a novel multidomain hyperthermophilic GH family 3 beta-glucosidase (TnBglB) from Thermotoga naphthophila RKU-10T (2018), Process Biochem., 66, 70-81 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 exprression in Escherichia coli Thermotoga petrophila

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.21 molecular modeling of structure Thermotoga petrophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 D-glucose
-
 Thermotoga petrophila
3.2.1.21 D-xylose
-
 Thermotoga petrophila
3.2.1.21 additional information not inhibitory: EDTA (10 mM), 1% of Urea, 2-mercaptoethanol, Triton X-100, and Tween-80 Thermotoga petrophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.45
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 1.11
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 1.34
-
 4-nitrophenyl beta-D-xylopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 2.35
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 2.95
-
 4-nitrophenyl alpha-D-fucopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 7.35
-
 cellobiose pH 5.0, 85°C Thermotoga petrophila

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ 10 mM, 175% of initial activity Thermotoga petrophila
3.2.1.21 Co2+ 10 mM, 110% of initial activity Thermotoga petrophila
3.2.1.21 Mn2+ 10 mM, 115% of initial activity Thermotoga petrophila
3.2.1.21 Zn2+ 10 mM, 120% of initial activity Thermotoga petrophila

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.21 Acetone 20% v/v, not inhibitory, 50% v/v, 50% loss of activity Thermotoga petrophila
3.2.1.21 acetonitrile 20% v/v, not inhibitory, 50% v/v, 54% loss of activity Thermotoga petrophila
3.2.1.21 dimethylformamide 20% v/v, not inhibitory, 50% v/v, 48% loss of activity Thermotoga petrophila
3.2.1.21 DMSO 20% v/v, not inhibitory, 50% v/v, 50% loss of activity Thermotoga petrophila
3.2.1.21 Ethanol 10%, 140% of initial activity Thermotoga petrophila
3.2.1.21 Ethyl acetate 20% v/v, not inhibitory, 50% v/v, 56% loss of activity Thermotoga petrophila
3.2.1.21 isopropanol 10%, 155% of initial activity Thermotoga petrophila
3.2.1.21 Methanol 10%, 148% of initial activity Thermotoga petrophila
3.2.1.21 n-Butanol 10%, 151% of initial activity Thermotoga petrophila

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermotoga petrophila D2C716
-
-
3.2.1.21 Thermotoga petrophila DSM 13996 D2C716
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 130000
-
 pH 5.0, 85°C Thermotoga petrophila

Storage Stability

EC Number Storage Stability Organism
3.2.1.21 4°C, purified, crude supernatant, and heat-treated enzyme remaine stable for 1 year Thermotoga petrophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl alpha-D-fucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-fucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermotoga petrophila 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + H2O
-
 Thermotoga petrophila DSM 13996 4-nitrophenol + cellobiose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermotoga petrophila DSM 13996 4-nitrophenol + D-galactopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermotoga petrophila DSM 13996 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga petrophila 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Thermotoga petrophila DSM 13996 4-nitrophenol + D-xylopyranose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermotoga petrophila 2 D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermotoga petrophila DSM 13996 2 D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 ? x * 81000, SDS-PAGE Thermotoga petrophila

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BglB
-
 Thermotoga petrophila
3.2.1.21 Tnap_0656
-
 Thermotoga petrophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 85
-
-
 Thermotoga petrophila

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 70 95 more than 75% of maximum activity Thermotoga petrophila

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 1469.8
-
 cellobiose pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 78809.5
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 83730.2
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 114048
-
 4-nitrophenyl alpha-D-fucopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 232540
-
 4-nitrophenyl beta-D-xylopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 1214290
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 85°C Thermotoga petrophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5
-
-
 Thermotoga petrophila

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 4 6
-
 Thermotoga petrophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 150
-
 D-glucose pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 200
-
 D-xylose pH 5.0, 85°C Thermotoga petrophila

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 200
-
 cellobiose pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 33536
-
 4-nitrophenyl beta-D-galactopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 38660
-
 4-nitrophenyl alpha-D-fucopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 75433
-
 4-nitrophenyl beta-D-cellobioside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 173537
-
 4-nitrophenyl beta-D-xylopyranoside pH 5.0, 85°C Thermotoga petrophila
3.2.1.21 2698410
-
 4-nitrophenyl beta-D-glucopyranoside pH 5.0, 85°C Thermotoga petrophila