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Literature summary extracted from

  • Ionita, E.; Gurgu, L.; Aprodu, I.; Stanciuc, N.; Dalmadi, I.; Bahrim, G.; Rapeanu, G.
    Characterization, purification, and temperature/pressure stability of polyphenol oxidase extracted from plums (Prunus domestica) (2017), Process Biochem., 56, 177-185 .
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.18.1 PPOextracted from plum shows stability at high pressure, with enzyme activation at 500 MPa Prunus domestica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 ascorbic acid 30% inhibition at 0.5 mM, complete inhibition at 5 mM Prunus domestica
1.14.18.1 Citric acid 24% inhibition at 0.5 mM, 32% inhibition at 5 mM Prunus domestica
1.14.18.1 EDTA 11% inhibition at 0.5 mM, 15% inhibition at 5 mM Prunus domestica
1.14.18.1 glycine 9% inhibition at 0.5 mM, 12% inhibition at 5 mM Prunus domestica
1.14.18.1 L-cysteine 13% inhibition at 0.5 mM, 38% inhibition at 5 mM Prunus domestica
1.14.18.1 additional information enzyme-inhibitor binding structure and inhibition mechanism, overview Prunus domestica
1.14.18.1 oxalic acid 16% inhibition at 0.5 mM, 31% inhibition at 5 mM Prunus domestica
1.14.18.1 sodium chloride 10% inhibition at 0.5 mM, 12% inhibition at 5 mM Prunus domestica
1.14.18.1 Sodium metabisulfite 13% inhibition at 0.5 mM, 38% inhibition at 5 mM Prunus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.18.1 2 4-methylcatechol + O2 Prunus domestica
-
 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 2 catechol + O2 Prunus domestica
-
 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 2 L-dopa + O2 Prunus domestica
-
 2 dopaquinone + 2 H2O
-
 ?
1.14.18.1 catechin + O2 Prunus domestica
-
 ? + 2 H2O
-
 ?
1.14.18.1 chlorogenic acid + O2 Prunus domestica
-
 ? + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Prunus domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1 native enzyme 32.81fold by ammonium sulfate fractionation, hydrophobic interaction chromatography, and anion exchange chromatography Prunus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 2 4-methylcatechol + O2
-
 Prunus domestica 2 4-methyl-1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 2 catechol + O2
-
 Prunus domestica 2 1,2-benzoquinone + 2 H2O
-
 ?
1.14.18.1 2 L-dopa + O2
-
 Prunus domestica 2 dopaquinone + 2 H2O
-
 ?
1.14.18.1 catechin + O2
-
 Prunus domestica ? + 2 H2O
-
 ?
1.14.18.1 chlorogenic acid + O2
-
 Prunus domestica ? + 2 H2O
-
 ?
1.14.18.1 additional information the enzyme shows no activity with caffeic acid, ferulic acid, 4-coumaric acid, p-cresol, and L-tyrosine Prunus domestica ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 ? x * 65000, SDS-PAGE Prunus domestica

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 polyphenol oxidase
-
 Prunus domestica
1.14.18.1 PPO
-
 Prunus domestica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.18.1 25
-
-
 Prunus domestica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 additional information
-
 the thermal inactivation follows a first-order kinetic model, with activation energy of Ea150.46 kJ/mol Prunus domestica
1.14.18.1 25 65 purified enzyme, high stability in the pH range Prunus domestica
1.14.18.1 60 85 enzyme inactivation kinetics, overview Prunus domestica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 6
-
-
 Prunus domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 5 7.5 over 50% activity within this range, reduced activity above and below Prunus domestica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 5 7 purified enzyme, high stability in the pH range Prunus domestica