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Literature summary extracted from

  • Wang, X.; Bai, Y.; Cai, Y.; Zheng, X.
    Biochemical characteristics of three feruloyl esterases with a broad substrate spectrum from Bacillus amyloliquefaciens H47 (2017), Process Biochem., 53, 109-115 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.42 EDTA 1 mM, 108% of initial activity Bacillus amyloliquefaciens
3.1.1.42 EDTA 1 mM, 111% of initial activity Bacillus amyloliquefaciens
3.1.1.42 EDTA 1 mM, 62.7% of initial activity Bacillus amyloliquefaciens
3.1.1.73 EDTA 1 mM, 108% of initial activity Bacillus amyloliquefaciens
3.1.1.73 EDTA 1 mM, 111% of initial activity Bacillus amyloliquefaciens
3.1.1.73 EDTA 1 mM, 62.7% of initial activity Bacillus amyloliquefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.42 Ca2+ 1 mM, 93% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Cu2+ 1 mM, 55.6% of initial activity; 1 mM, 57.6% of initial activity; 1 mM, 8.5% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Fe2+ 1 mM, 61% of initial activity; 1 mM, 71% of initial activity; 1 mM, 84% of initial activity Bacillus amyloliquefaciens
3.1.1.42 K+ 1 mM, 88% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Mg2+ 1 mM, 92% of initial activity; 1 mM, 94% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Mn2+ 1 mM, 83% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Na+ 1 mM, 95% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Ni2+ 1 mM, 15.1% of initial activity; 1 mM, 44.5% of initial activity; 1 mM, 63% of initial activity Bacillus amyloliquefaciens
3.1.1.42 PMSF 1 mM, 15% of initial activity; 1 mM, 55% of initial activity; 1 mM, 60% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Zn2+ 1 mM, 14.3% of initial activity; 1 mM, 67.7% of initial activity; 1 mM, 74% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Ca2+ 1 mM, 93% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Cu2+ 1 mM, 55.6% of initial activity; 1 mM, 57.6% of initial activity; 1 mM, 8.5% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Fe2+ 1 mM, 61% of initial activity; 1 mM, 71% of initial activity; 1 mM, 84% of initial activity Bacillus amyloliquefaciens
3.1.1.73 K+ 1 mM, 88% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Mg2+ 1 mM, 92% of initial activity; 1 mM, 94% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Mn2+ 1 mM, 83% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Na+ 1 mM, 95% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Ni2+ 1 mM, 15.1% of initial activity; 1 mM, 44.5% of initial activity; 1 mM, 63% of initial activity Bacillus amyloliquefaciens
3.1.1.73 PMSF 1 mM, 15% of initial activity; 1 mM, 55% of initial activity; 1 mM, 60% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Zn2+ 1 mM, 14.3% of initial activity; 1 mM, 67.7% of initial activity; 1 mM, 74% of initial activity Bacillus amyloliquefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.42 1.51
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.42 4.79
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.42 6.06
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 0.49
-
 methyl ferulate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 0.58
-
 ethyl ferulate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 0.78
-
 methyl p-coumarate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 0.86
-
 ethyl ferulate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 1.14
-
 methyl ferulate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 1.42
-
 methyl p-coumarate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 1.51
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 1.81
-
 methyl p-coumarate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 1.99
-
 methyl ferulate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 2.15
-
 ethyl ferulate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 4.79
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 6.06
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.42 Ca2+ 1 mM, 107% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Co2+ 1 mM, 115% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Co2+ 1 mM, 107% of initial activity Bacillus amyloliquefaciens
3.1.1.42 K+ 1 mM, 115% of initial activity Bacillus amyloliquefaciens
3.1.1.42 K+ 1 mM, 105% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Mg2+ 1 mM, 143% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Mn2+ 1 mM, 113% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Na+ 1 mM, 120% of initial activity Bacillus amyloliquefaciens
3.1.1.42 Na+ 1 mM, 151% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Ca2+ 1 mM, 107% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Co2+ 1 mM, 115% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Co2+ 1 mM, 107% of initial activity Bacillus amyloliquefaciens
3.1.1.73 K+ 1 mM, 115% of initial activity Bacillus amyloliquefaciens
3.1.1.73 K+ 1 mM, 105% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Mg2+ 1 mM, 143% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Mn2+ 1 mM, 113% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Na+ 1 mM, 120% of initial activity Bacillus amyloliquefaciens
3.1.1.73 Na+ 1 mM, 151% of initial activity Bacillus amyloliquefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.42 Bacillus amyloliquefaciens A0A1B1WA13 isoform Fae04, cf. EC 3.1.1.73
-
3.1.1.42 Bacillus amyloliquefaciens A0A1B1WA14 isoform Fae06, cf. EC 3.1.1.73
-
3.1.1.42 Bacillus amyloliquefaciens ANW61885 isoform Fae09, cf. EC 3.1.1.73
-
3.1.1.42 Bacillus amyloliquefaciens H47 A0A1B1WA13 isoform Fae04, cf. EC 3.1.1.73
-
3.1.1.42 Bacillus amyloliquefaciens H47 A0A1B1WA14 isoform Fae06, cf. EC 3.1.1.73
-
3.1.1.42 Bacillus amyloliquefaciens H47 ANW61885 isoform Fae09, cf. EC 3.1.1.73
-
3.1.1.73 Bacillus amyloliquefaciens A0A1B1WA13 isoform Fae04
-
3.1.1.73 Bacillus amyloliquefaciens A0A1B1WA14 isoform Fae06
-
3.1.1.73 Bacillus amyloliquefaciens ANW61885 isoform Fae09, cf. EC 3.1.1.42
-
3.1.1.73 Bacillus amyloliquefaciens H47 A0A1B1WA13 isoform Fae04
-
3.1.1.73 Bacillus amyloliquefaciens H47 A0A1B1WA14 isoform Fae06
-
3.1.1.73 Bacillus amyloliquefaciens H47 ANW61885 isoform Fae09, cf. EC 3.1.1.42
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.42 chlorogenate + H2O
-
 Bacillus amyloliquefaciens caffeate + quinate
-
 ?
3.1.1.42 chlorogenate + H2O
-
 Bacillus amyloliquefaciens H47 caffeate + quinate
-
 ?
3.1.1.42 additional information enzyme additionally acts as feruloyl esterase, cleaving ethyl and methyl esters of hydroxycinnamic acis, reaction of EC 3.1.1.73 Bacillus amyloliquefaciens ?
-
 ?
3.1.1.42 additional information enzyme additionally acts as feruloyl esterase, cleaving ethyl and methyl esters of hydroxycinnamic acis, reaction of EC 3.1.1.73 Bacillus amyloliquefaciens H47 ?
-
 ?
3.1.1.73 chlorogenate + H2O
-
 Bacillus amyloliquefaciens caffeate + quinate
-
 ?
3.1.1.73 chlorogenate + H2O
-
 Bacillus amyloliquefaciens H47 caffeate + quinate
-
 ?
3.1.1.73 cinnamic acid benzyl ester + H2O
-
 Bacillus amyloliquefaciens cinnamate + benzyl alcohol
-
 ?
3.1.1.73 ethyl caffeate + H2O
-
 Bacillus amyloliquefaciens ethanol + caffeate
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Bacillus amyloliquefaciens ethanol + ferulate
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Bacillus amyloliquefaciens H47 ethanol + ferulate
-
 ?
3.1.1.73 geranyl acetate + H2O
-
 Bacillus amyloliquefaciens geraniol + acetate
-
 ?
3.1.1.73 geranyl acetate + H2O
-
 Bacillus amyloliquefaciens H47 geraniol + acetate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Bacillus amyloliquefaciens methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Bacillus amyloliquefaciens methanol + p-coumarate
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Bacillus amyloliquefaciens methanol + sinapinate
-
 ?
3.1.1.73 phenethyl caffeate + H2O
-
 Bacillus amyloliquefaciens phenylethanol + caffeate
-
 ?
3.1.1.73 vinyl benzoate + H2O
-
 Bacillus amyloliquefaciens vinyl alcohol + benzoate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.42 ? x * 31000, SDS-PAGE Bacillus amyloliquefaciens
3.1.1.42 ? x * 30000, SDS-PAGE Bacillus amyloliquefaciens
3.1.1.42 ? x * 29000, SDS-PAGE Bacillus amyloliquefaciens
3.1.1.73 ? x * 31000, SDS-PAGE Bacillus amyloliquefaciens
3.1.1.73 ? x * 30000, SDS-PAGE Bacillus amyloliquefaciens
3.1.1.73 ? x * 29000, SDS-PAGE Bacillus amyloliquefaciens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.42 KX091144
-
 Bacillus amyloliquefaciens
3.1.1.73 KX091144
-
 Bacillus amyloliquefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.42 30
-
-
 Bacillus amyloliquefaciens
3.1.1.42 35
-
-
 Bacillus amyloliquefaciens
3.1.1.42 40
-
-
 Bacillus amyloliquefaciens
3.1.1.73 30
-
-
 Bacillus amyloliquefaciens
3.1.1.73 35
-
-
 Bacillus amyloliquefaciens
3.1.1.73 40
-
-
 Bacillus amyloliquefaciens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.42 60
-
 60% residual activity Bacillus amyloliquefaciens
3.1.1.73 60
-
 60% residual activity Bacillus amyloliquefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.42 55
-
 complete inactivation Bacillus amyloliquefaciens
3.1.1.42 60
-
 complete inactivation Bacillus amyloliquefaciens
3.1.1.73 55
-
 complete inactivation Bacillus amyloliquefaciens
3.1.1.73 60
-
 complete inactivation Bacillus amyloliquefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.42 6.52
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.42 12.06
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.42 275
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 0.34
-
 methyl ferulate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 1.05
-
 ethyl ferulate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 2.2
-
 methyl p-coumarate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 3.18
-
 ethyl ferulate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 6.52
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 8.6
-
 methyl ferulate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 8.9
-
 methyl p-coumarate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 12
-
 methyl ferulate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 12.06
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 25
-
 ethyl ferulate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 43.88
-
 methyl p-coumarate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 275
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.42 8
-
-
 Bacillus amyloliquefaciens
3.1.1.73 8
-
-
 Bacillus amyloliquefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.42 3 5
-
 Bacillus amyloliquefaciens
3.1.1.42 8 9
-
 Bacillus amyloliquefaciens
3.1.1.73 3 5
-
 Bacillus amyloliquefaciens
3.1.1.73 8 9
-
 Bacillus amyloliquefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.42 2.52
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.42 4.32
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.42 45.31
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 0.69
-
 methyl ferulate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 1.21
-
 methyl p-coumarate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 1.23
-
 ethyl ferulate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 2.52
-
 chlorogenate pH 8.0, 40°C Bacillus amyloliquefaciens
3.1.1.73 4.32
-
 methyl ferulate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 4.32
-
 chlorogenate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 5.48
-
 ethyl ferulate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 10.57
-
 methyl ferulate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 11.4
-
 methyl p-coumarate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 11.72
-
 ethyl ferulate pH 8.0, 30°C Bacillus amyloliquefaciens
3.1.1.73 30.9
-
 methyl p-coumarate pH 8.0, 35°C Bacillus amyloliquefaciens
3.1.1.73 45.31
-
 chlorogenate pH 8.0, 30°C Bacillus amyloliquefaciens