Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kumar, A.; Kaachra, A.; Bhardwaj, S.; Kumar, S.
    Copper, zinc superoxide dismutase of Curcuma aromatica is a kinetically stable protein (2014), Process Biochem., 49, 1288-1296 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.15.1.1 DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli Curcuma aromatica

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.15.1.1 2-mercaptoethanol 17% inhibition at 3 mM Curcuma aromatica
1.15.1.1 DTT 13% inhibition at 2.5 mM Curcuma aromatica
1.15.1.1 EDTA 15% inhibition at 0.5 mM Curcuma aromatica
1.15.1.1 guanidinium hydrochloride 40% inhibition at 2 M, 85% at 5 M Curcuma aromatica
1.15.1.1 H2O2 58% inhibition at 2 mM Curcuma aromatica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.15.1.1 additional information
-
additional information Michaelis–Menten and Lineweaver–Burk kinetics Curcuma aromatica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.15.1.1 Cu2+ a Cu,Zn SOD Curcuma aromatica
1.15.1.1 Zn2+ a Cu,Zn SOD Curcuma aromatica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+ Curcuma aromatica
-
O2 + H2O2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.15.1.1 Curcuma aromatica E6Y8N8 collected at 10:00 am from the experimental farm of the Institute of Himalayan Bioresource Technology at Palampur, Distt. Kangra, India
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.15.1.1 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and dialysis Curcuma aromatica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.15.1.1 leaf
-
Curcuma aromatica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.15.1.1 1250
-
purified recombinant His-tagged enzyme, pH 7.8, 37°C, substrate riboflavin Curcuma aromatica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.15.1.1 2 superoxide + 2 H+
-
Curcuma aromatica O2 + H2O2
-
?

Subunits

EC Number Subunits Comment Organism
1.15.1.1 homodimer 2 * 17500, about, sequence calculation, 2 * 18800, about, SDS-PAGE Curcuma aromatica

Synonyms

EC Number Synonyms Comment Organism
1.15.1.1 Ca-Cu,Zn SOD
-
Curcuma aromatica
1.15.1.1 Cu,Zn superoxide dismutase
-
Curcuma aromatica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.15.1.1 20
-
-
Curcuma aromatica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.15.1.1
-
80 activity range between -10-80°C Curcuma aromatica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.15.1.1 70.45
-
midpoint of thermal transition (Tm) Curcuma aromatica
1.15.1.1 121
-
Ca-Cu Z- SOD retains 50% of the maximum activity after autoclaving, autoclaved Ca-Cu,Zn SOD exhibits 40% of maximum activity of unautoclaved enzyme at 20°C and 30°C. Thermal inactivation kinetics of Ca-Cu,Zn SOD, overview Curcuma aromatica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.15.1.1 7.8
-
-
Curcuma aromatica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.15.1.1 6 9 activity range Curcuma aromatica

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.15.1.1 3 10 Ca–Cu,Zn SOD is stable at a wide storage pH ranging from pH 3 to pH 10. After24 h of incubation, the enzyme retains more than 80% of maximal activity in the pH range of pH 3.0-9.0, whereas at pH 10.0 the enzyme loses 36.21% of maximal activity, profile overview Curcuma aromatica