BRENDA - Enzyme Database

Purification and properties of reductase of the three-component p-cymene methyl hydroxylase from Pseudomonas chlororaphis subsp. aureofaciens

Dutta, T.; Dutta, A.; Chakraborty, J.; Sarkar, J.; Pal Chowdhury, P.; Gunsalus, I.; Process Biochem. 47, 1263-1267 (2012)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.25
-
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
-
Pseudomonas chlororaphis subsp. aureofaciens
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.25
39000
-
gel fitlration, reductase component CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
39000
-
gel fitlration
Pseudomonas chlororaphis subsp. aureofaciens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.25
Pseudomonas chlororaphis subsp. aureofaciens
P95461 and O84920 and O84919
P95461 i.e. reductase subunit CymA, cf. EC 1.8.1.3, O84920 i.e. subunit CymB, O84919 i.e. subunit CymM
-
1.18.1.3
Pseudomonas chlororaphis subsp. aureofaciens
P95461
enzyme acts as reductase subunit of p-cymene mono-oxygenase, EC 1.14.15.25
-
Storage Stability
EC Number
Storage Stability
Organism
1.14.15.25
70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month
Pseudomonas chlororaphis subsp. aureofaciens
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.25
?
x * 38433, calculated, x * 38000, SDS-PAGE of reductase component CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
?
x * 38433, calculated, x * 38000, SDS-PAGE
Pseudomonas chlororaphis subsp. aureofaciens
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.15.25
30
-
10 min, 42% loss of activity, NADH-cytochrome c reductase activity of subunit CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.14.15.25
50
-
10 min, 98% loss of activity, NADH-cytochrome c reductase activity of subunit CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
30
-
10 min, 42% loss of activity
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
50
-
10 min, 98% loss of activity
Pseudomonas chlororaphis subsp. aureofaciens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.25
8.4
-
NADH-cytochrome c reductase activity of subunit CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
8.4
-
-
Pseudomonas chlororaphis subsp. aureofaciens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.3
FAD
1.07 mol/mol of enzyme
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
additional information
no activity with NADPH
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
NADH
-
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
[2Fe-2S]-center
presence of one [2Fe-2S] center per enzyme molecule
Pseudomonas chlororaphis subsp. aureofaciens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.25
-
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
-
Pseudomonas chlororaphis subsp. aureofaciens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.3
FAD
1.07 mol/mol of enzyme
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
additional information
no activity with NADPH
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
NADH
-
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
[2Fe-2S]-center
presence of one [2Fe-2S] center per enzyme molecule
Pseudomonas chlororaphis subsp. aureofaciens
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.14.15.25
39000
-
gel fitlration, reductase component CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
39000
-
gel fitlration
Pseudomonas chlororaphis subsp. aureofaciens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.15.25
70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
70°C, 50 mM potassium phosphate buffer (pH 7.0), 60% glycerol (v/v), subunit CymA is stable for 1 month
Pseudomonas chlororaphis subsp. aureofaciens
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.25
?
x * 38433, calculated, x * 38000, SDS-PAGE of reductase component CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
?
x * 38433, calculated, x * 38000, SDS-PAGE
Pseudomonas chlororaphis subsp. aureofaciens
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.14.15.25
30
-
10 min, 42% loss of activity, NADH-cytochrome c reductase activity of subunit CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.14.15.25
50
-
10 min, 98% loss of activity, NADH-cytochrome c reductase activity of subunit CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
30
-
10 min, 42% loss of activity
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
50
-
10 min, 98% loss of activity
Pseudomonas chlororaphis subsp. aureofaciens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.25
8.4
-
NADH-cytochrome c reductase activity of subunit CymA
Pseudomonas chlororaphis subsp. aureofaciens
1.18.1.3
8.4
-
-
Pseudomonas chlororaphis subsp. aureofaciens
Expression
EC Number
Organism
Commentary
Expression
1.14.15.25
Pseudomonas chlororaphis subsp. aureofaciens
expression of subunits CymABM is induced by growth on p-cymene
up
1.18.1.3
Pseudomonas chlororaphis subsp. aureofaciens
expression of subunits CymABM is induced by growth on p-cymene
up
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.15.25
Pseudomonas chlororaphis subsp. aureofaciens
expression of subunits CymABM is induced by growth on p-cymene
up
1.18.1.3
Pseudomonas chlororaphis subsp. aureofaciens
expression of subunits CymABM is induced by growth on p-cymene
up