BRENDA - Enzyme Database

Carnitine metabolism to trimethylamine by an unusual Rieske-type oxygenase from human microbiota

Zhu, Y.; Jameson, E.; Crosatti, M.; Schaefer, H.; Rajakumar, K.; Bugg, T.D.; Chen, Y.; Proc. Natl. Acad. Sci. USA 111, 4268-4273 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.239
expressed in Escherichia coli BLR(DE3) pLysS cells
Acinetobacter baumannii
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.13.239
E205A
inactive
Acinetobacter baumannii
1.14.13.239
E205D
inactive
Acinetobacter baumannii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.239
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
1.14.13.239
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.239
Acinetobacter baumannii
D0C9N6 AND D0C9N8
alpha subunit and beta subunit
-
1.14.13.239
Acinetobacter baumannii ATCC19606
D0C9N6 AND D0C9N8
alpha subunit and beta subunit
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.13.239
His-tag affinity column chromatography
Acinetobacter baumannii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.239
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
1.14.13.239
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.239
FAD
-
Acinetobacter baumannii
1.14.13.239
NAD+
-
Acinetobacter baumannii
1.14.13.239
NADH
-
Acinetobacter baumannii
1.14.13.239
NADPH
-
Acinetobacter baumannii
1.14.13.239
[2Fe-2S]-center
-
Acinetobacter baumannii
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.239
expressed in Escherichia coli BLR(DE3) pLysS cells
Acinetobacter baumannii
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.239
FAD
-
Acinetobacter baumannii
1.14.13.239
NAD+
-
Acinetobacter baumannii
1.14.13.239
NADH
-
Acinetobacter baumannii
1.14.13.239
NADPH
-
Acinetobacter baumannii
1.14.13.239
[2Fe-2S]-center
-
Acinetobacter baumannii
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.13.239
E205A
inactive
Acinetobacter baumannii
1.14.13.239
E205D
inactive
Acinetobacter baumannii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.239
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
1.14.13.239
L-carnitine + NADH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
Acinetobacter baumannii ATCC19606
-
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.239
His-tag affinity column chromatography
Acinetobacter baumannii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.239
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
1.14.13.239
L-carnitine + NADH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD+ + H2O
-
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?
1.14.13.239
L-carnitine + NADPH + H+ + O2
-
746297
Acinetobacter baumannii ATCC19606
(3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NADP+ + H2O
-
-
-
?