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Literature summary extracted from

  • Liu, Y.; Nakamura, A.; Nakazawa, Y.; Asano, N.; Ford, K.A.; Hohn, M.J.; Tanaka, I.; Yao, M.; Soell, D.
    Ancient translation factor is essential for tRNA-dependent cysteine biosynthesis in methanogenic archaea (2014), Proc. Natl. Acad. Sci. USA, 111, 10520-10525 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.27 expressed in Escherichia coli Rosetta 2(DE3) cells Methanococcus maripaludis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.27 0.0116
-
 tRNACys at pH 7.5 and 60°C Methanococcus maripaludis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.27 270000
-
 gel filtration Methanococcus maripaludis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys Methanococcus maripaludis
-
 AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.27 Methanococcus maripaludis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.27 TALON metal affinity resin column chromatography Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.27 ATP + O-phospho-L-serine + tRNACys
-
 Methanococcus maripaludis AMP + diphosphate + O-phospho-L-seryl-tRNACys
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.27 homotetramer 4 * 60000, SDS-PAGE Methanococcus maripaludis

Synonyms

EC Number Synonyms Comment Organism
6.1.1.27 phosphoseryl-tRNA synthetase
-
 Methanococcus maripaludis
6.1.1.27 SepRS
-
 Methanococcus maripaludis
6.1.1.27 sepS
-
 Methanococcus maripaludis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.27 0.07
-
 tRNACys at pH 7.5 and 60°C Methanococcus maripaludis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.27 ATP
-
 Methanococcus maripaludis