BRENDA - Enzyme Database

Protein complexing in a methanogen suggests electron bifurcation and electron delivery from formate to heterodisulfide reductase

Costa, K.; Wong, P.; Wang, T.; Lie, T.; Dodsworth, J.; Swanson, I.; Burn, J.; Hackett, M.; Leigh, J.; Proc. Natl. Acad. Sci. USA 107, 11050-11055 (2010)

Data extracted from this reference:

Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.6
CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
Methanococcus maripaludis
-
formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.98.6
Methanococcus maripaludis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.98.6
Ni2+ resin column chromatography
Methanococcus maripaludis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.6
CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
746283
Methanococcus maripaludis
formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
-
?
1.8.98.6
additional information
the protein complex consists of heterodisulfide reductase, formylmethanofuran dehydrogenase, F420-nonreducing hydrogenase, and formate dehydrogenase. Either H2 or formate can donate electrons to the heterodisulfide-H2 via F420-nonreducing hydrogenase or formate via formate dehydrogenase. When H2 is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase via F420-nonreducing hydrogenase. Flavin-mediated electron bifurcation at heterodisulfide reductase A then results in reduction of the CoM-S-S-CoB heterodisulfide and a ferredoxin that is used by formylmethanofuran dehydrogenase for the first step in methanogenesis. When hydrogen is limiting or is replaced by formate, formate dehydrogenase is highly expressed and incorporates into the complex. When formate is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase from formate via formate dehydrogenase
746283
Methanococcus maripaludis
?
-
-
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.98.6
CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
Methanococcus maripaludis
-
formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.98.6
Ni2+ resin column chromatography
Methanococcus maripaludis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.98.6
CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
-
746283
Methanococcus maripaludis
formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
-
-
-
?
1.8.98.6
additional information
the protein complex consists of heterodisulfide reductase, formylmethanofuran dehydrogenase, F420-nonreducing hydrogenase, and formate dehydrogenase. Either H2 or formate can donate electrons to the heterodisulfide-H2 via F420-nonreducing hydrogenase or formate via formate dehydrogenase. When H2 is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase via F420-nonreducing hydrogenase. Flavin-mediated electron bifurcation at heterodisulfide reductase A then results in reduction of the CoM-S-S-CoB heterodisulfide and a ferredoxin that is used by formylmethanofuran dehydrogenase for the first step in methanogenesis. When hydrogen is limiting or is replaced by formate, formate dehydrogenase is highly expressed and incorporates into the complex. When formate is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase from formate via formate dehydrogenase
746283
Methanococcus maripaludis
?
-
-
-
-