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Literature summary extracted from

  • Costa, K.; Wong, P.; Wang, T.; Lie, T.; Dodsworth, J.; Swanson, I.; Burn, J.; Hackett, M.; Leigh, J.
    Protein complexing in a methanogen suggests electron bifurcation and electron delivery from formate to heterodisulfide reductase (2010), Proc. Natl. Acad. Sci. USA, 107, 11050-11055 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.98.6 CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+ Methanococcus maripaludis
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 formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
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Organism

EC Number Organism UniProt Comment Textmining
1.8.98.6 Methanococcus maripaludis
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.6 Ni2+ resin column chromatography Methanococcus maripaludis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.6 CO2 + reduced ferredoxin [iron-sulfur] cluster + CoB + CoM + H+
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 Methanococcus maripaludis formate + oxidized ferredoxin [iron-sulfur] cluster + CoM-S-S-CoB
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 ?
1.8.98.6 additional information the protein complex consists of heterodisulfide reductase, formylmethanofuran dehydrogenase, F420-nonreducing hydrogenase, and formate dehydrogenase. Either H2 or formate can donate electrons to the heterodisulfide-H2 via F420-nonreducing hydrogenase or formate via formate dehydrogenase. When H2 is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase via F420-nonreducing hydrogenase. Flavin-mediated electron bifurcation at heterodisulfide reductase A then results in reduction of the CoM-S-S-CoB heterodisulfide and a ferredoxin that is used by formylmethanofuran dehydrogenase for the first step in methanogenesis. When hydrogen is limiting or is replaced by formate, formate dehydrogenase is highly expressed and incorporates into the complex. When formate is used as the electron donor for methanogenesis, electrons are transferred to heterodisulfide reductase from formate via formate dehydrogenase Methanococcus maripaludis ?
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