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Literature summary extracted from
- Biosynthesis of gamma-butyrolactone autoregulators that switch on secondary metabolism and morphological development in Streptomyces (2007), Proc. Natl. Acad. Sci. USA, 104, 2378-2383 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.1.277 | expression in Escherichia coli | Streptomyces griseus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.277 | additional information | EDTA (5 mM) has no effect on the reaction | Streptomyces griseus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.277 | 0.2046 | - |
glycerone phosphate | pH 7.5, 15°C | Streptomyces griseus |  |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.1.277 | additional information | no metals are required for the activity | Streptomyces griseus |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.277 | 37700 | - |
SDS-PAGE | Streptomyces griseus |
| 2.3.1.277 | 45400 | - |
gel filtration | Streptomyces griseus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.1.277 | a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | Streptomyces griseus | AfsA is the key enzyme for A factor biosynthesis | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
| 2.3.1.277 | a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | Streptomyces griseus IFO13350 | AfsA is the key enzyme for A factor biosynthesis | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.3.1.113 | Streptomyces griseus | - |
- |
- |
| 2.3.1.277 | Streptomyces griseus | P18394 | - |
- |
| 2.3.1.277 | Streptomyces griseus IFO13350 | P18394 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.1.277 | - |
Streptomyces griseus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.3.1.113 | additional information | no activity with NADH | Streptomyces griseus | ? | - |
? | |
| 1.3.1.113 | [4-(6-methylheptanoyl)-5-oxo-2,5-dihydrofuran-3-yl]methyl phosphate + NADPH + H+ | - |
Streptomyces griseus | [(3S)-4-(6-methylheptanoyl)-5-oxooxolan-3-yl]methyl phosphate + NADP+ | ? | ? | |
| 2.3.1.277 | a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | - |
Streptomyces griseus | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
| 2.3.1.277 | a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | AfsA is the key enzyme for A factor biosynthesis | Streptomyces griseus | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
| 2.3.1.277 | a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | - |
Streptomyces griseus IFO13350 | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
| 2.3.1.277 | a medium-chain 3-oxoacyl-[acyl-carrier protein] + glycerone phosphate | AfsA is the key enzyme for A factor biosynthesis | Streptomyces griseus IFO13350 | 2-oxo-3-(phosphooxy)propyl 3-oxoalkanoate + a holo-[acyl-carrier protein] | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.3.1.113 | ? | x * 32000, SDS-PAGE | Streptomyces griseus |
| 2.3.1.277 | monomer | 1 * 37700, SDS-PAGE | Streptomyces griseus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.3.1.113 | bprA | - |
Streptomyces griseus |
| 1.3.1.113 | butenolide phosphate reductase | - |
Streptomyces griseus |
| 2.3.1.277 | afsA | - |
Streptomyces griseus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.277 | 15 | - |
- |
Streptomyces griseus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.277 | 15 | 30 | at 30°C, the activity decreases to 32% of that measured at 15°C, and almost no activity is detected at 35°C | Streptomyces griseus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.1.277 | 2 | - |
glycerone phosphate | pH 7.5, 15°C | Streptomyces griseus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.3.1.277 | 6.5 | 7.5 | Trisx01HCl buffer | Streptomyces griseus |
| 2.3.1.277 | 7 | 8 | sodium phosphate-citrate buffer | Streptomyces griseus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.3.1.113 | NADPH | - |
Streptomyces griseus | |
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Release 2023.1 (January 2023)
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