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Literature summary extracted from

  • Equbal, A.; Suman, S.S.; Anwar, S.; Singh, K.P.; Zaidi, A.; Sardar, A.H.; Das, P.; Ali, V.
    Stage-dependent expression and up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani (2014), PLoS ONE, 9, e97600 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.9 gene tryS, DNA and amino acid sequence determination and analysis, sequence comparisons, real-time PCR expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.9 cytosol subcellular localization study, overview Leishmania donovani 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.9 Mg2+ required Leishmania donovani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.9 glutathione + glutathionylspermidine + ATP Leishmania donovani
-
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + glutathionylspermidine + ATP Leishmania donovani MHOM / IN / 80 / Dd8
-
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + glutathionylspermidine + ATP Leishmania donovani MHOM / IN / 83 / Ag
-
N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP Leishmania donovani
-
glutathionylspermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP Leishmania donovani MHOM / IN / 80 / Dd8
-
glutathionylspermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP Leishmania donovani MHOM / IN / 83 / Ag
-
glutathionylspermidine + ADP + phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.9 Leishmania donovani Q8IFU8
-
-
6.3.1.9 Leishmania donovani MHOM / IN / 80 / Dd8 Q8IFU8
-
-
6.3.1.9 Leishmania donovani MHOM / IN / 83 / Ag Q8IFU8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.9 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by tag cleavage though thrombin, the tag is removed by benzamidine affinity chromatography, the purified enzyme is dialysed Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.9 additional information stage-dependent expression and up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani. LdTryS is constitutively transcribed in the exponential as well as the stationary phase, the latter shows higher enzyme expression Leishmania donovani
-
6.3.1.9 promastigote LdTryS is overexpressed 2fold in Amp B resistant and stationary phase promastigotes compared to sensitive strain and logarithmic phase, respectively Leishmania donovani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
Leishmania donovani N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
Leishmania donovani MHOM / IN / 80 / Dd8 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
Leishmania donovani MHOM / IN / 83 / Ag N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP
-
Leishmania donovani glutathionylspermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP
-
Leishmania donovani MHOM / IN / 80 / Dd8 glutathionylspermidine + ADP + phosphate
-
?
6.3.1.9 glutathione + spermidine + ATP
-
Leishmania donovani MHOM / IN / 83 / Ag glutathionylspermidine + ADP + phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.9 LdTryS
-
Leishmania donovani
6.3.1.9 TryS
-
Leishmania donovani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.9 30
-
assay at Leishmania donovani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.9 8 8.5
-
Leishmania donovani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.3.1.9 6 10 activity range, profile overview Leishmania donovani

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.9 ATP
-
Leishmania donovani

Expression

EC Number Organism Comment Expression
6.3.1.9 Leishmania donovani H2O2 treatment up to 0.150 mM for 8 hrs leads to 2fold increased expression of LdTryS probably to cope up with oxidative stress generated by H2O2. Stage-dependent up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani up

General Information

EC Number General Information Comment Organism
6.3.1.9 metabolism trypanothione synthetase (TryS) of thiol metabolic pathway is the sole enzyme responsible for the biosynthesis of trypanothione in Leishmania donovani Leishmania donovani
6.3.1.9 physiological function the enzyme is involved in Amp B resistance and during oxidative stress to help the parasites survival Leishmania donovani