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Literature summary extracted from

  • Alperstein, A.; Ulrich, B.; Garofalo, D.M.; Dreisbach, R.; Raff, H.; Sheppard, K.
    The predatory bacterium Bdellovibrio bacteriovorus aspartyl-tRNA synthetase recognizes tRNAAsn as a substrate (2014), PLoS ONE, 9, e110842 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.23 expressed in Escherichia coli BL21(DE3) cells Bdellovibrio bacteriovorus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.23 0.0014
-
tRNAAsp at pH 7.2 and 37°C Bdellovibrio bacteriovorus
6.1.1.23 0.0014
-
tRNAAsn at pH 7.2 and 37°C Bdellovibrio bacteriovorus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.23 ATP + L-aspartate + tRNAAsp Bdellovibrio bacteriovorus
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
6.1.1.23 ATP + L-aspartate + tRNAAsp Bdellovibrio bacteriovorus HD100
-
AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.23 Bdellovibrio bacteriovorus Q6MI59
-
-
6.1.1.23 Bdellovibrio bacteriovorus HD100 Q6MI59
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.23 nickel-affinity column chromatography Bdellovibrio bacteriovorus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.23 ATP + L-aspartate + tRNAAsn
-
Bdellovibrio bacteriovorus AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
6.1.1.23 ATP + L-aspartate + tRNAAsn
-
Bdellovibrio bacteriovorus HD100 AMP + diphosphate + L-aspartyl-tRNAAsn
-
?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
Bdellovibrio bacteriovorus AMP + diphosphate + L-aspartyl-tRNAAsp
-
?
6.1.1.23 ATP + L-aspartate + tRNAAsp
-
Bdellovibrio bacteriovorus HD100 AMP + diphosphate + L-aspartyl-tRNAAsp
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.23 Aspartyl-tRNA synthetase
-
Bdellovibrio bacteriovorus
6.1.1.23 AspRS
-
Bdellovibrio bacteriovorus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.23 0.17
-
tRNAAsn at pH 7.2 and 37°C Bdellovibrio bacteriovorus
6.1.1.23 0.52
-
tRNAAsp at pH 7.2 and 37°C Bdellovibrio bacteriovorus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.23 ATP
-
Bdellovibrio bacteriovorus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.23 120
-
tRNAAsn at pH 7.2 and 37°C Bdellovibrio bacteriovorus
6.1.1.23 400
-
tRNAAsp at pH 7.2 and 37°C Bdellovibrio bacteriovorus