BRENDA - Enzyme Database

Identification and characterization of a new erythromycin biosynthetic gene cluster in Actinopolyspora erythraea YIM90600, a novel erythronolide-producing halophilic actinomycete isolated from salt field

Chen, D.; Feng, J.; Huang, L.; Zhang, Q.; Wu, J.; Zhu, X.; Duan, Y.; Xu, Z.; PLoS ONE 9, e108129 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.13.188
gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001
Saccharopolyspora erythraea
1.14.13.188
gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001)
Actinopolyspora erythraea
1.14.15.35
gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001
Saccharopolyspora erythraea
1.14.15.35
gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001)
Actinopolyspora erythraea
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.14.13.188
additional information
construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression
Saccharopolyspora erythraea
1.14.15.35
additional information
construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression
Saccharopolyspora erythraea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Saccharopolyspora erythraea
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Actinopolyspora erythraea
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Actinopolyspora erythraea
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
erythronolide H + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Saccharopolyspora erythraea NRRL2338
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Actinopolyspora erythraea YIM90600
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
additional information
Actinopolyspora erythraea
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
1.14.13.188
additional information
Actinopolyspora erythraea YIM90600
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Saccharopolyspora erythraea
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Saccharopolyspora erythraea NRRL2338
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea YIM90600
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea YIM90600
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
additional information
Actinopolyspora erythraea
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
1.14.15.35
additional information
Actinopolyspora erythraea YIM90600
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.13.188
Actinopolyspora erythraea
A0A099D4T8
halophilic actinomycete isolated from Baicheng salt field
-
1.14.13.188
Actinopolyspora erythraea YIM90600
A0A099D4T8
halophilic actinomycete isolated from Baicheng salt field
-
1.14.13.188
Saccharopolyspora erythraea
Q00441
-
-
1.14.13.188
Saccharopolyspora erythraea NRRL2338
Q00441
-
-
1.14.15.35
Actinopolyspora erythraea
A0A099D4T8
halophilic actinomycete isolated from Baicheng salt field
-
1.14.15.35
Actinopolyspora erythraea YIM90600
A0A099D4T8
halophilic actinomycete isolated from Baicheng salt field
-
1.14.15.35
Saccharopolyspora erythraea
Q00441
-
-
1.14.15.35
Saccharopolyspora erythraea NRRL2338
Q00441
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea NRRL2338
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea YIM90600
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
746251
Actinopolyspora erythraea
erythronolide H + NADP+ + H2O
-
-
-
?
1.14.13.188
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea
?
-
-
-
-
1.14.13.188
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea YIM90600
?
-
-
-
-
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea NRRL2338
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea YIM90600
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
746251
Actinopolyspora erythraea
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
746251
Actinopolyspora erythraea YIM90600
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea
?
-
-
-
-
1.14.15.35
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea YIM90600
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.188
30
-
assay at
Actinopolyspora erythraea
1.14.13.188
30
-
assay at
Saccharopolyspora erythraea
1.14.15.35
30
-
assay at
Actinopolyspora erythraea
1.14.15.35
30
-
assay at
Saccharopolyspora erythraea
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.188
7.5
-
assay at
Actinopolyspora erythraea
1.14.13.188
7.5
-
assay at
Saccharopolyspora erythraea
1.14.15.35
7.5
-
assay at
Actinopolyspora erythraea
1.14.15.35
7.5
-
assay at
Saccharopolyspora erythraea
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.188
cytochrome P450
-
Actinopolyspora erythraea
1.14.13.188
cytochrome P450
-
Saccharopolyspora erythraea
1.14.13.188
Ferredoxin
-
Actinopolyspora erythraea
1.14.13.188
Ferredoxin
-
Saccharopolyspora erythraea
1.14.13.188
NADPH
-
Actinopolyspora erythraea
1.14.13.188
NADPH
-
Saccharopolyspora erythraea
1.14.15.35
cytochrome P450
-
Actinopolyspora erythraea
1.14.15.35
cytochrome P450
-
Saccharopolyspora erythraea
1.14.15.35
Ferredoxin
-
Actinopolyspora erythraea
1.14.15.35
Ferredoxin
-
Saccharopolyspora erythraea
1.14.15.35
ferredoxin [iron-sulfur] cluster
-
Actinopolyspora erythraea
1.14.15.35
ferredoxin [iron-sulfur] cluster
-
Saccharopolyspora erythraea
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.13.188
gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001
Saccharopolyspora erythraea
1.14.13.188
gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001)
Actinopolyspora erythraea
1.14.15.35
gene eryF, genetic structure, sequence comparison, recombinant expression of His6-tagged enzyme in Saccharopolyspora erythraea strain ZL2001
Saccharopolyspora erythraea
1.14.15.35
gene eryF, whole-genome sequencing, DNA and amino acid sequence determination and analysis, genetic structure,sequence comparison, genomic DNA of Saccharopolyspora erythraea strain ZL2001 serves as template for PCR amplification, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression in and complementation fo Saccharopolyspora erythraea eryF-deletion mutant strain EX105(ZL2001)
Actinopolyspora erythraea
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.13.188
cytochrome P450
-
Actinopolyspora erythraea
1.14.13.188
cytochrome P450
-
Saccharopolyspora erythraea
1.14.13.188
Ferredoxin
-
Actinopolyspora erythraea
1.14.13.188
Ferredoxin
-
Saccharopolyspora erythraea
1.14.13.188
NADPH
-
Actinopolyspora erythraea
1.14.13.188
NADPH
-
Saccharopolyspora erythraea
1.14.15.35
cytochrome P450
-
Actinopolyspora erythraea
1.14.15.35
cytochrome P450
-
Saccharopolyspora erythraea
1.14.15.35
Ferredoxin
-
Actinopolyspora erythraea
1.14.15.35
Ferredoxin
-
Saccharopolyspora erythraea
1.14.15.35
ferredoxin [iron-sulfur] cluster
-
Actinopolyspora erythraea
1.14.15.35
ferredoxin [iron-sulfur] cluster
-
Saccharopolyspora erythraea
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.14.13.188
additional information
construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression
Saccharopolyspora erythraea
1.14.15.35
additional information
construction of an erythronolide H-producing Saccharopolyspora erythraea mutant via gene complementation is not successful. Construction of en eryF deletion mutant strainEX105 from strain ZL2001 and complementation by Actinopolyspora erythraea eryF expression
Saccharopolyspora erythraea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Saccharopolyspora erythraea
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Actinopolyspora erythraea
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Actinopolyspora erythraea
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
erythronolide H + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Saccharopolyspora erythraea NRRL2338
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
Actinopolyspora erythraea YIM90600
C-6 hydroxylation
erythronolide B + NADP+ + H2O
-
-
?
1.14.13.188
additional information
Actinopolyspora erythraea
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
1.14.13.188
additional information
Actinopolyspora erythraea YIM90600
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Saccharopolyspora erythraea
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Saccharopolyspora erythraea NRRL2338
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea YIM90600
C-6 hydroxylation
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
Actinopolyspora erythraea YIM90600
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.15.35
additional information
Actinopolyspora erythraea
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
1.14.15.35
additional information
Actinopolyspora erythraea YIM90600
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea NRRL2338
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea YIM90600
erythronolide B + NADP+ + H2O
-
-
-
?
1.14.13.188
6-deoxyerythronolide B + NADPH + H+ + O2
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
746251
Actinopolyspora erythraea
erythronolide H + NADP+ + H2O
-
-
-
?
1.14.13.188
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea
?
-
-
-
-
1.14.13.188
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea YIM90600
?
-
-
-
-
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Saccharopolyspora erythraea NRRL2338
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
C-6 hydroxylation
746251
Actinopolyspora erythraea YIM90600
erythronolide B + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
746251
Actinopolyspora erythraea
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
6-deoxyerythronolide B + reduced ferredoxin [iron-sulfur] cluster + H+ + O2
a two-step oxidation (14-hydroxylation and 6,18-epoxyidation) via the intermediate erythronolide B
746251
Actinopolyspora erythraea YIM90600
erythronolide H + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.35
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea
?
-
-
-
-
1.14.15.35
additional information
the enzyme plays a role as a C-12 hydroxylase, rather than a C-14 hydroxylase of the erythronolide in vitro
746251
Actinopolyspora erythraea YIM90600
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.13.188
30
-
assay at
Actinopolyspora erythraea
1.14.13.188
30
-
assay at
Saccharopolyspora erythraea
1.14.15.35
30
-
assay at
Actinopolyspora erythraea
1.14.15.35
30
-
assay at
Saccharopolyspora erythraea
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.13.188
7.5
-
assay at
Actinopolyspora erythraea
1.14.13.188
7.5
-
assay at
Saccharopolyspora erythraea
1.14.15.35
7.5
-
assay at
Actinopolyspora erythraea
1.14.15.35
7.5
-
assay at
Saccharopolyspora erythraea
General Information
EC Number
General Information
Commentary
Organism
1.14.13.188
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH) and erythronolide I are proposed. Formation of erythronolide H is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B
Actinopolyspora erythraea
1.14.13.188
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG, correlation of genotype and chemotype
Saccharopolyspora erythraea
1.14.13.188
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Actinopolyspora erythraea
1.14.13.188
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Saccharopolyspora erythraea
1.14.15.35
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH) and erythronolide I are proposed. Formation of erythronolide H is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B
Actinopolyspora erythraea
1.14.15.35
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG, correlation of genotype and chemotype
Saccharopolyspora erythraea
1.14.15.35
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Actinopolyspora erythraea
1.14.15.35
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Saccharopolyspora erythraea
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.13.188
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH) and erythronolide I are proposed. Formation of erythronolide H is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B
Actinopolyspora erythraea
1.14.13.188
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG, correlation of genotype and chemotype
Saccharopolyspora erythraea
1.14.13.188
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Actinopolyspora erythraea
1.14.13.188
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Saccharopolyspora erythraea
1.14.15.35
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG. By correlating genotype and chemotype, the biosynthetic pathways of 3'-demethyl-erythromycin C, erythronolide H (EH) and erythronolide I are proposed. Formation of erythronolide H is supposed to be sequentially biosynthesized via C-6/C-18 epoxidation and C-14 hydroxylation from 6-deoxyerythronolide B
Actinopolyspora erythraea
1.14.15.35
evolution
the Er gene cluster from Actinopolyspora erythraea strain YIM90600 shares high identity and similarity with the one of Saccharopolyspora erythraea strain NRRL2338, except for two absent genes, eryBI and eryG, correlation of genotype and chemotype
Saccharopolyspora erythraea
1.14.15.35
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Actinopolyspora erythraea
1.14.15.35
metabolism
the ezyme is involved in the biosynthetic pathway of the final erythronolide product, erythronolid A, pathway overview
Saccharopolyspora erythraea