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Literature summary extracted from

  • Silvestro, D.; Andersen, T.G.; Schaller, H.; Jensen, P.E.
    Plant sterol metabolism. DELTA7-Sterol-C5-desaturase (STE1/DWARF7), DELTA5,7-sterol-DELTA7-reductase (DWARF5) and DELTA24-sterol-DELTA24-reductase (DIMINUTO/DWARF1) show multiple subcellular localizations in Arabidopsis thaliana (Heynh) L (2013), PLoS ONE, 8, e56429 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.72 recombinant expression of C-terminally YFP-tagged wild-type enzyme in Arabidopsis thaliana mutant lines deficient in the enzyme in the endoplasmic reticulum and plasma membrane Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.72 additional information construction of a diminuto/dwarf1 (dim) T-DNA insertion mutant. The dim mutant plants, similarly to the dwarf5-2, show a dwarf phenotype due to the deficiency in brassinosteroids, caused by a block in the D24-sterol-D24-reductase activity. Compared to the wild-type, the dim plants show reduced levels of campesterol and sitosterol associated to an increase of 24-methylene cholesterol and isofucosterol, their respective metabolic precursors and substrates of DIM, complementation experiments with dwarf5-2, dim, and ste1-1 Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.72 endoplasmic reticulum
-
 Arabidopsis thaliana 5783
-
1.3.1.72 plasma membrane
-
 Arabidopsis thaliana 5886
-

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.72 Arabidopsis thaliana Q39085
-
-
1.3.1.72 Arabidopsis thaliana Col-0 Q39085
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.72 DELTA5,7-avenasterol + NADPH + H+
-
 Arabidopsis thaliana DELTA5,7-sitosterol + NADP+
-
 ?
1.3.1.72 DELTA5,7-avenasterol + NADPH + H+
-
 Arabidopsis thaliana Col-0 DELTA5,7-sitosterol + NADP+
-
 ?
1.3.1.72 DELTA7-avenasterol + NADPH + H+
-
 Arabidopsis thaliana DELTA7-sitosterol + NADP+
-
 ?
1.3.1.72 DELTA7-avenasterol + NADPH + H+
-
 Arabidopsis thaliana Col-0 DELTA7-sitosterol + NADP+
-
 ?
1.3.1.72 ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H+
-
 Arabidopsis thaliana ergosterol + NADP+
-
 ?
1.3.1.72 ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADPH + H+
-
 Arabidopsis thaliana Col-0 ergosterol + NADP+
-
 ?
1.3.1.72 isofucosterol + NADPH + H+
-
 Arabidopsis thaliana sitosterol + NADP+
-
 ?
1.3.1.72 isofucosterol + NADPH + H+
-
 Arabidopsis thaliana Col-0 sitosterol + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.72 DELTA24-sterol-DELTA24-reductase
-
 Arabidopsis thaliana
1.3.1.72 DIM
-
 Arabidopsis thaliana
1.3.1.72 DIMINUTO/DWARF1
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.72 NADPH
-
 Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
1.3.1.72 malfunction the enzyme dim mutant plants, similarly to the dwarf5-2, show a dwarf phenotype due to the deficiency in brassinosteroids, caused by a block in the D24-sterol-D24-reductase activity. Compared to the wild-type, the dim plants show reduced levels of campesterol and sitosterol associated to an increase of 24-methylene cholesterol and isofucosterol, their respective metabolic precursors and substrates of DIM. Mutant dim seedlings have short hypocotyls, petioles, and roots. Leaves of dim are round, curly, and dark green in color. In adult flowering plants, dim shows extremely short inflorescences with small flowers and severely reduced fertility. Phenotype, overview Arabidopsis thaliana
1.3.1.72 metabolism the enzyme is involved in the sterol biosynthetic pathway in Arabidopsis thaliana, overview Arabidopsis thaliana