Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Garba, L.; Mohamad Ali, M.S.; Oslan, S.N.; Rahman, R.N.
    Molecular cloning and functional expression of a DELTA9-fatty acid desaturase from an antarctic Pseudomonas sp. A3 (2016), PLoS ONE, 11, e0160681 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.28 gene PA3FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli, GCMS analysis reveals that palmitoleic (C16:1DELTA9) and oleic acids (C18:1DELTA9) are the only unsaturated fatty acids identified in addition to the endogenous fatty acids, expression at 15°C Pseudomonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.28 membrane according to transmembrane helices prediction, the protein has two hydrophobic domains located between amino acids 10-32 and 135-157 and thought to be long enough to span the membrane bilayer two times with the two protein termini facing the cytosol Pseudomonas sp. 16020
-
1.14.19.28 additional information sequence analysis reveals a potential N-terminal signal peptide Pseudomonas sp.
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.28 1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp.
-
 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp. A3(2015c)
-
 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp.
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+ Pseudomonas sp. A3(2015c)
-
 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.28 Pseudomonas sp. A0A0S1Z203
-
-
1.14.19.28 Pseudomonas sp. A3(2015c) A0A0S1Z203
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.28 1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. A3(2015c) 1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?
1.14.19.28 1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
 Pseudomonas sp. A3(2015c) 1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.28 ? x * 45000, about, sequence calculation Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.14.19.28 DELTA9-fatty acid desaturase
-
 Pseudomonas sp.
1.14.19.28 DELTA9-fatty acid desaturase-like protein
-
 Pseudomonas sp.
1.14.19.28 More cf. EC 1.14.19.27 Pseudomonas sp.
1.14.19.28 PA3FAD9
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.28 Ferredoxin
-
 Pseudomonas sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.19.28 Pseudomonas sp. sequence calculation
-
 9.48