BRENDA - Enzyme Database

Molecular cloning and functional expression of a DELTA9-fatty acid desaturase from an antarctic Pseudomonas sp. A3

Garba, L.; Mohamad Ali, M.S.; Oslan, S.N.; Rahman, R.N.; PLoS ONE 11, e0160681 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.28
gene PA3FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli, GCMS analysis reveals that palmitoleic (C16:1DELTA9) and oleic acids (C18:1DELTA9) are the only unsaturated fatty acids identified in addition to the endogenous fatty acids, expression at 15°C
Pseudomonas sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.28
membrane
according to transmembrane helices prediction, the protein has two hydrophobic domains located between amino acids 10-32 and 135-157 and thought to be long enough to span the membrane bilayer two times with the two protein termini facing the cytosol
Pseudomonas sp.
16020
-
1.14.19.28
additional information
sequence analysis reveals a potential N-terminal signal peptide
Pseudomonas sp.
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A3(2015c)
-
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A3(2015c)
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.28
Pseudomonas sp.
A0A0S1Z203
-
-
1.14.19.28
Pseudomonas sp. A3(2015c)
A0A0S1Z203
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp.
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp. A3(2015c)
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp.
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp. A3(2015c)
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 45000, about, sequence calculation
Pseudomonas sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.28
Ferredoxin
-
Pseudomonas sp.
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.19.28
Pseudomonas sp.
sequence calculation
-
9.48
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.28
gene PA3FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, functional recombinant expression in Escherichia coli, GCMS analysis reveals that palmitoleic (C16:1DELTA9) and oleic acids (C18:1DELTA9) are the only unsaturated fatty acids identified in addition to the endogenous fatty acids, expression at 15°C
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.28
Ferredoxin
-
Pseudomonas sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.28
membrane
according to transmembrane helices prediction, the protein has two hydrophobic domains located between amino acids 10-32 and 135-157 and thought to be long enough to span the membrane bilayer two times with the two protein termini facing the cytosol
Pseudomonas sp.
16020
-
1.14.19.28
additional information
sequence analysis reveals a potential N-terminal signal peptide
Pseudomonas sp.
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A3(2015c)
-
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A3(2015c)
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp.
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-palmitoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp. A3(2015c)
1-palmitoleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp.
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
746210
Pseudomonas sp. A3(2015c)
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 45000, about, sequence calculation
Pseudomonas sp.
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.19.28
Pseudomonas sp.
sequence calculation
-
9.48