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Literature summary extracted from

  • Ojo, K.K.; Ranade, R.M.; Zhang, Z.; Dranow, D.M.; Myers, J.B.; Choi, R.; Nakazawa Hewitt, S.; Edwards, T.E.; Davies, D.R.; Lorimer, D.; Boyle, S.M.; Barrett, L.K.; Buckner, F.S.; Fan, E.; Van Voorhis, W.C.
    Brucella melitensis methionyl-tRNA-synthetase (MetRS), a potential drug target for brucellosis (2016), PLoS ONE, 11, e0160350 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 expressed in Escherichia coli Rosetta 2(DE3) cells Brucella melitensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.10 enzyme in complex with inhibitors, sitting drop vapor diffusion method, using 0.2 M ammonium nitrate, 20% (wv)PEG-3350 pH 7.5 or 25% (w/v) PEG-1500, 100 mM MMT buffer, pH 5.0 or 25% (w/v) PEG-1500, 7% (w/v) PCB pH 4.0 or 0.2 Mammonium acetate, 0.1 M sodium acetate trihydrate, pH 4.6, 30% (w/v) PEG-4000 Brucella melitensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.10 2-[(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)amino]quinolin-4(1H)-one
-
 Brucella melitensis
6.1.1.10 N-(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)-N'-thiophen-3-ylurea
-
 Brucella melitensis
6.1.1.10 N-(3-[[(3-chloro-5-methoxyphenyl)methyl]amino]propyl)-N'-phenylurea
-
 Brucella melitensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Brucella melitensis
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet Brucella melitensis 2308
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Brucella melitensis Q2YQ76 biovar Abortus
-
6.1.1.10 Brucella melitensis 2308 Q2YQ76 biovar Abortus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 Ni-NTA column chromatography and Superdex 75 gel filtration Brucella melitensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Brucella melitensis AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Brucella melitensis 2308 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 methionyl-tRNA-synthetase
-
 Brucella melitensis
6.1.1.10 MetRS
-
 Brucella melitensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.10 60.6
-
 melting temperature of unbound apoenzyme Brucella melitensis

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Brucella melitensis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.1.1.10 0.000006
-
 at pH 7.9 and 37°C Brucella melitensis 2-[(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)amino]quinolin-4(1H)-one
6.1.1.10 0.000272
-
 at pH 7.9 and 37°C Brucella melitensis N-(3-[[(3,5-dichlorophenyl)methyl]amino]propyl)-N'-thiophen-3-ylurea
6.1.1.10 0.001657
-
 at pH 7.9 and 37°C Brucella melitensis N-(3-[[(3-chloro-5-methoxyphenyl)methyl]amino]propyl)-N'-phenylurea