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Literature summary extracted from

  • Gu, L.; Hitzel, J.; Moll, F.; Kruse, C.; Malik, R.A.; Preussner, J.; Looso, M.; Leisegang, M.S.; Steinhilber, D.; Brandes, R.P.; Fork, C.
    The histone demethylase PHF8 is essential for endothelial cell migration (2016), PLoS ONE, 11, e0146645 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.65 gene PHF8, quantitative RT-PCR enzyme expression analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.65 additional information PHF8 enzyme knockout by PHF8 siRNA Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.65 nucleus the expression of PHF8 is restricted to the nucleus Homo sapiens 5634
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.65 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
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 histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H4 N6-methyl-L-lysine20 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H4 L-lysine20 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 additional information Homo sapiens PHF8 demethylates H3K9me1, and if overexpressed also of H3K9me2 and H4K20me1 in endothelial cells, whereas H3K27me1/2 and H3K4me3, the latter being thought to act as anchor for PHF8, are not affected ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.65 Homo sapiens Q9UPP1
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.65 endothelial cell
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 Homo sapiens
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1.14.11.65 HEK-293T cell
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 Homo sapiens
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1.14.11.65 HMEC-1 cell
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 Homo sapiens
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1.14.11.65 HUVEC cell
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 Homo sapiens
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1.14.11.65 umbilical vein endothelial cell
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 Homo sapiens
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.65 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H3 N6-methyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 histone H4 N6-methyl-L-lysine20 + 2-oxoglutarate + O2
-
 Homo sapiens histone H4 L-lysine20 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 additional information PHF8 demethylates H3K9me1, and if overexpressed also of H3K9me2 and H4K20me1 in endothelial cells, whereas H3K27me1/2 and H3K4me3, the latter being thought to act as anchor for PHF8, are not affected Homo sapiens ?
-
 ?
1.14.11.65 additional information PHF8 demethylates H3K9me1, and if overexpressed also of H3K9me2 and H4K20me1 in endothelial cells, whereas H3K27me1/2 and H3K4me3 are not affected Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.65 histone demethylase
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 Homo sapiens
1.14.11.65 PHF8
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 Homo sapiens
1.14.11.65 plant homeodomain finger protein 8
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 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.65 evolution the enzyme PHF8 is a member of the KDM7 family Homo sapiens
1.14.11.65 malfunction overexpression of PHF8 catalyzes the removal of methyl-groups from histone 3 lysine 9 (H3K9) and H4K20, whereas knockdown of the enzyme increases H3K9 methylation. Knockdown of PHF8 by RNAi also attenuates endothelial proliferation and survival. Enzyme knockout by PHF8 siRNA attenuates the capacity for migration and developing of capillary-like structures Homo sapiens
1.14.11.65 physiological function the histone demethylase PHF8 is essential for endothelial cell migration and regulates brain and craniofacial development. Enzyme PHF8 controls the endothelial gene repressor E2F4. PHF8 maintains E2F4 but not E2F1 expression in endothelial cells. Chromatin immunoprecipitation reveals that PHF8 reduces the H3K9me2 level at the E2F4 transcriptional start site, demonstrating a direct function of PHF8 in endothelial E2F4 gene regulation. PHF8, by controlling E2F4 expression, maintains endothelial function Homo sapiens
1.14.11.65 physiological function overexpression of PHF8 catalyzes the removal of methyl-groups from histone 3 lysine 9 (H3K9) and H4K20, whereas knockdown of the enzyme increases H3K9 methylation. Knockdown of PHF8 also attenuates endothelial proliferation and survival. PHF8 knockdown attenuates the capacity for migration and developing of capillary-like structures. Gene repressor E2F4 is controlled by PHF8. PHF8 maintains E2F4 but not E2F1 expression in endothelial cells and reduces the H3K9me2 level at the E2F4 transcriptional start site Homo sapiens