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Literature summary extracted from

  • Chang, S.; Berman, J.; Sheng, Y.; Wang, Y.; Capell, T.; Shi, L.; Ni, X.; Sandmann, G.; Christou, P.; Zhu, C.
    Cloning and functional characterization of the maize (Zea mays L.) carotenoid epsilon hydroxylase gene (2015), PLoS ONE, 10, e0128758 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.158 expression of CYP97C19 cDNA under the control of the constitutive CaMV 35S promoter in the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene Zea mays
1.14.14.158 gene CYP97C19, cDNA is cloned from leaves, the ZmCYP97C19 gene has nine introns and ten exons, DNA and amino acid sequence determination and analysis, sequence comparisons, genetic structure, overview. Functional recombinant expression under the control of constitutive CaMV 35S promoter in rosettes leaves of the Arabidopsis thaliana lut1 knockout mutant, which lacks a functional CYP97C1 (LUT1) gene. Lutein accumulates to high levels in the rosette leaves of the transgenic lines but not in the untransformed lut1 mutants, carotenoid extraction and quantification from Arabidopsis thaliana lut1-CYP97C1 leaves Zea mays

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.158 zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 Zea mays maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.158 zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 Zea mays L. cv B73 maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.158 Zea mays K9J9Z8
-
-
1.14.14.158 Zea mays K9J9Z8 cv. B73
-
1.14.14.158 Zea mays L. cv B73 K9J9Z8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.158 endosperm
-
 Zea mays
-
1.14.14.158 leaf
-
 Zea mays
-
1.14.14.158 rosette leaf
-
 Zea mays
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.158 zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity Zea mays lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.158 zeinoxanthin + [reduced NADPH-hemoprotein reductase] + O2 maize CYP97C19 is an enzyme with strong zeinoxanthin epsilon-ring hydroxylation activity Zea mays L. cv B73 lutein + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.158 ? x * 61900, about, sequence calculation of ZmCYP97C19 Zea mays

Synonyms

EC Number Synonyms Comment Organism
1.14.14.158 carotenoid epsilon hydroxylase
-
 Zea mays
1.14.14.158 carotenoid epsilon-hydroxylase
-
 Zea mays
1.14.14.158 CYP97C
-
 Zea mays
1.14.14.158 CYP97C19
-
 Zea mays
1.14.14.158 heme-containing cytochrome P450 carotene epsilon-ring hydroxylase
-
 Zea mays
1.14.14.158 LUT1
-
 Zea mays

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.158 cytochrome P450
-
 Zea mays
1.14.14.158 heme
-
 Zea mays