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Literature summary extracted from
- Methanesulfonate (MSA) catabolic genes from marine and estuarine bacteria (2015), PLoS ONE, 10, e0125735 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.111 | gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha | Candidatus Filomicrobium marinum |
| 1.14.13.111 | gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha | Methylobacterium sp. P1 |
| 1.14.13.111 | gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha | Methylobacterium sp. RD4.1 |
| 1.14.13.111 | gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha | Hyphomicrobium sp. P2 |
| 1.14.13.111 | gene msmA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain DH5alpha | Methylosulfonomonas methylovora |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.111 | cytoplasm | - |
Candidatus Filomicrobium marinum | 5737 | - |
| 1.14.13.111 | cytoplasm | - |
Methylobacterium sp. P1 | 5737 | - |
| 1.14.13.111 | cytoplasm | - |
Methylobacterium sp. RD4.1 | 5737 | - |
| 1.14.13.111 | cytoplasm | - |
Hyphomicrobium sp. P2 | 5737 | - |
| 1.14.13.111 | cytoplasm | - |
Methylosulfonomonas methylovora | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.111 | Fe2+ | two iron ions per enzyme molecule | Candidatus Filomicrobium marinum |  |
| 1.14.13.111 | Fe2+ | two iron ions per enzyme molecule | Methylobacterium sp. P1 | |
| 1.14.13.111 | Fe2+ | two iron ions per enzyme molecule | Methylobacterium sp. RD4.1 | |
| 1.14.13.111 | Fe2+ | two iron ions per enzyme molecule | Hyphomicrobium sp. P2 | |
| 1.14.13.111 | Fe2+ | two iron ions per enzyme molecule | Methylosulfonomonas methylovora | |
| 1.14.13.111 | [2Fe-2S] cluster | the predicted gene product of gene msmA reveals a unique sequence in the region associated to the Rieske-type [2Fe-2S] cluster, with a longer-than-usual 26-amino acid spacer between the two highly conserved cysteine-histidine groups in the CXH-Xn-CXXH conserved motif | Methylosulfonomonas methylovora | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Candidatus Filomicrobium marinum | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Methylobacterium sp. P1 | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Methylobacterium sp. RD4.1 | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Hyphomicrobium sp. P2 | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Methylosulfonomonas methylovora | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Candidatus Filomicrobium marinum W | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Methylosulfonomonas methylovora M2 | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | Candidatus Filomicrobium marinum Y | - |
formaldehyde + NAD+ + sulfite + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.111 | Candidatus Filomicrobium marinum | A0A0D6JGB8 | alpha-subunit of the hydroxylase; from ocean surface water sample collected roughly 5 Km off the coast of Matosinhos, Portugal | - |
| 1.14.13.111 | Candidatus Filomicrobium marinum | A0A0G2RHR1 | from ocean surface water sample collected roughly 5 Km off the coast of Matosinhos, Portugal | - |
| 1.14.13.111 | Candidatus Filomicrobium marinum W | A0A0G2RHR1 | from ocean surface water sample collected roughly 5 Km off the coast of Matosinhos, Portugal | - |
| 1.14.13.111 | Candidatus Filomicrobium marinum Y | A0A0D6JGB8 | alpha-subunit of the hydroxylase; from ocean surface water sample collected roughly 5 Km off the coast of Matosinhos, Portugal | - |
| 1.14.13.111 | Hyphomicrobium sp. P2 | A0A0G2RHB5 | - |
- |
| 1.14.13.111 | Methylobacterium sp. P1 | A0A0G2RG68 | - |
- |
| 1.14.13.111 | Methylobacterium sp. RD4.1 | A0A0G2RI26 | - |
- |
| 1.14.13.111 | Methylosulfonomonas methylovora | Q9X404 | alpha-subunit of the hydroxylase | - |
| 1.14.13.111 | Methylosulfonomonas methylovora M2 | Q9X404 | alpha-subunit of the hydroxylase | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Candidatus Filomicrobium marinum | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Methylobacterium sp. P1 | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Methylobacterium sp. RD4.1 | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Hyphomicrobium sp. P2 | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Methylosulfonomonas methylovora | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Candidatus Filomicrobium marinum W | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Methylosulfonomonas methylovora M2 | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
| 1.14.13.111 | methanesulfonate + NADH + H+ + O2 | - |
Candidatus Filomicrobium marinum Y | formaldehyde + NAD+ + sulfite + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.111 | methanesulfonate monooxygenase | - |
Candidatus Filomicrobium marinum |
| 1.14.13.111 | methanesulfonate monooxygenase | - |
Methylobacterium sp. P1 |
| 1.14.13.111 | methanesulfonate monooxygenase | - |
Methylobacterium sp. RD4.1 |
| 1.14.13.111 | methanesulfonate monooxygenase | - |
Hyphomicrobium sp. P2 |
| 1.14.13.111 | methanesulfonate monooxygenase | - |
Methylosulfonomonas methylovora |
| 1.14.13.111 | MSAMO | - |
Candidatus Filomicrobium marinum |
| 1.14.13.111 | MSAMO | - |
Methylobacterium sp. P1 |
| 1.14.13.111 | MSAMO | - |
Methylobacterium sp. RD4.1 |
| 1.14.13.111 | MSAMO | - |
Hyphomicrobium sp. P2 |
| 1.14.13.111 | MSAMO | - |
Methylosulfonomonas methylovora |
| 1.14.13.111 | MsmA | - |
Candidatus Filomicrobium marinum |
| 1.14.13.111 | MsmA | - |
Methylobacterium sp. P1 |
| 1.14.13.111 | MsmA | - |
Methylobacterium sp. RD4.1 |
| 1.14.13.111 | MsmA | - |
Hyphomicrobium sp. P2 |
| 1.14.13.111 | MsmA | - |
Methylosulfonomonas methylovora |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.13.111 | evolution | the two msm operons in Filomicrobium sp. strains Y and W are divergently transcribed, like in Methylosulfonomonas methylovora str. M2 | Candidatus Filomicrobium marinum |
| 1.14.13.111 | physiological function | the enzyme is responsible for splitting the C-S bond, catalyzing the first oxidative step of MSA to the central methylotrophic intermediate formaldehyde with the release of sulfite, which is subsequently oxidized to sulfate. Formaldehyde is assimilated through the serine cycle or fully oxidized to CO2 and H2O, in order to yield reducing power and energy | Candidatus Filomicrobium marinum |
| 1.14.13.111 | physiological function | the enzyme is responsible for splitting the C-S bond, catalyzing the first oxidative step of MSA to the central methylotrophic intermediate formaldehyde with the release of sulfite, which is subsequently oxidized to sulfate. Formaldehyde is assimilated through the serine cycle or fully oxidized to CO2 and H2O, in order to yield reducing power and energy | Methylobacterium sp. P1 |
| 1.14.13.111 | physiological function | the enzyme is responsible for splitting the C-S bond, catalyzing the first oxidative step of MSA to the central methylotrophic intermediate formaldehyde with the release of sulfite, which is subsequently oxidized to sulfate. Formaldehyde is assimilated through the serine cycle or fully oxidized to CO2 and H2O, in order to yield reducing power and energy | Methylobacterium sp. RD4.1 |
| 1.14.13.111 | physiological function | the enzyme is responsible for splitting the C-S bond, catalyzing the first oxidative step of MSA to the central methylotrophic intermediate formaldehyde with the release of sulfite, which is subsequently oxidized to sulfate. Formaldehyde is assimilated through the serine cycle or fully oxidized to CO2 and H2O, in order to yield reducing power and energy | Hyphomicrobium sp. P2 |
| 1.14.13.111 | physiological function | the enzyme is responsible for splitting the C-S bond, catalyzing the first oxidative step of MSA to the central methylotrophic intermediate formaldehyde with the release of sulfite, which is subsequently oxidized to sulfate. Formaldehyde is assimilated through the serine cycle or fully oxidized to CO2 and H2O, in order to yield reducing power and energy | Methylosulfonomonas methylovora |
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