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Literature summary extracted from

  • Benitez, D.; Medeiros, A.; Fiestas, L.; Panozzo-Zenere, E.A.; Maiwald, F.; Prousis, K.C.; Roussaki, M.; Calogeropoulou, T.; Detsi, A.; Jaeger, T.; Sarlauskas, J.; Peterlin Masic, L.; Kunick, C.; Labadie, G.R.; Flohe, L.; Comini, M.A.
    Identification of novel chemical scaffolds inhibiting trypanothione synthetase from pathogenic trypanosomatids (2016), PLoS Negl. Trop. Dis., 10, e0004617 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.1.9 1,4,7-trioxo-1lambda5,4lambda5,7lambda5-benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,5]oxadiazole activates, 118.8% activity Trypanosoma brucei brucei
6.3.1.9 1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one activates, 107.1% activity Trypanosoma cruzi
6.3.1.9 1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one activates, 166.3% activity Trypanosoma brucei brucei
6.3.1.9 1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one activates, 126.7% activity Trypanosoma brucei brucei
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-hydroxy-3-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one activates, 119.1% activity Trypanosoma brucei brucei
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one activates, 119.0% activity Trypanosoma brucei brucei
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(3,4-dimethoxybenzoyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one activates, 123.2% activity Trypanosoma brucei brucei
6.3.1.9 4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-1-(6-methoxy-1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one activates, 116.4% activity Trypanosoma brucei brucei
6.3.1.9 4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1-(6-methyl-1,3-benzothiazol-2-yl)-1,5-dihydro-2H-pyrrol-2-one activates, 109.7% activity Trypanosoma cruzi
6.3.1.9 4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1-(6-methyl-1,3-benzothiazol-2-yl)-1,5-dihydro-2H-pyrrol-2-one activates, 128.2% activity Trypanosoma brucei brucei
6.3.1.9 6-fluoro-3-oxo-4,5-didehydro-2,1,3lambda5-benzoxadiazole activates, 118.8% activity Trypanosoma cruzi
6.3.1.9 8-[(2E)-2-[(2,4-dihydroxyphenyl)methylidene]hydrazinyl]-7-(2-hydroxy-3-phenoxypropyl)-1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione activates, 161.6% activity Trypanosoma cruzi
6.3.1.9 J20 slightly stimulating Leishmania infantum
6.3.1.9 additional information no effect by 1H-purine-2,6(3H,7H)-dione derivatives (Z)-8-(2-(2,4-dihydroxybenzylidene)hydrazinyl)-7-(2-hydroxy-3-phenoxy propyl)-1,3-dimethyl-1H-purine-2,6(3H,7H)-dione and 6-fluoro-3-oxo-4,5-didehydro-2,1,3lambda5-benzoxadiazole Trypanosoma brucei brucei
6.3.1.9 [3-[4-([6-(3,4-dimethoxyphenyl)-7-[(ethylcarbamothioyl)amino]pyrido[2,3-d]pyrimidin-2-yl]amino)phenyl]propyl]molybdenum(1+) activates, 168.6% activity Trypanosoma cruzi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.9 gene TRS, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strains BL21(DE3) or Tuner (DE3) Trypanosoma cruzi
6.3.1.9 gene tryS, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strains BL21(DE3) or Tuner (DE3) Leishmania infantum
6.3.1.9 gene tryS, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strains BL21(DE3) or Tuner (DE3) Trypanosoma brucei brucei

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.9 (Z)-8-(2-(2,4-dihydroxybenzylidene)hydrazinyl)-7-(2-hydroxy-3-phenoxy propyl)-1,3-dimethyl-1H-purine-2,6(3H,7H)-dione 88.5% activity remaining Leishmania infantum
6.3.1.9 1,4,7-trioxo-1lambda5,4lambda5,7lambda5-benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,5]oxadiazole 64.4% activity remaining Trypanosoma cruzi
6.3.1.9 1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 83.5% activity remaining Leishmania infantum
6.3.1.9 1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one 83.5% activity remaining Leishmania infantum
6.3.1.9 1-(1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one 90.8% activity remaining Trypanosoma cruzi
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-hydroxy-3-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 85.3% activity remaining Leishmania infantum
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-hydroxy-3-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 97.4% activity remaining Trypanosoma cruzi
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 82.1% activity remaining Leishmania infantum
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 98.7% activity remaining Trypanosoma cruzi
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(3,4-dimethoxybenzoyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one 66.0% activity remaining Leishmania infantum
6.3.1.9 1-(6-chloro-1,3-benzothiazol-2-yl)-4-(3,4-dimethoxybenzoyl)-5-(4-fluorophenyl)-3-hydroxy-1,5-dihydro-2H-pyrrol-2-one 99.6% activity remaining Trypanosoma cruzi
6.3.1.9 2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
-
 Leishmania infantum
6.3.1.9 2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide 59.0% activity remaining Trypanosoma brucei brucei
6.3.1.9 2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide 55.5% activity remaining Trypanosoma cruzi
6.3.1.9 4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-1-(6-methoxy-1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 77.9% activity remaining Leishmania infantum
6.3.1.9 4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-1-(6-methoxy-1,3-benzothiazol-2-yl)-5-(4-methoxyphenyl)-1,5-dihydro-2H-pyrrol-2-one 97.6% activity remaining Trypanosoma cruzi
6.3.1.9 4-(2,3-dihydro-1,4-benzodioxine-6-carbonyl)-3-hydroxy-5-(4-methoxyphenyl)-1-(6-methyl-1,3-benzothiazol-2-yl)-1,5-dihydro-2H-pyrrol-2-one 83.9% activity remaining Leishmania infantum
6.3.1.9 5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
-
 Leishmania infantum
6.3.1.9 5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole 7.3% activity remaining Trypanosoma brucei brucei
6.3.1.9 5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole 63.5% activity remaining Trypanosoma cruzi
6.3.1.9 6-fluoro-3-oxo-4,5-didehydro-2,1,3lambda5-benzoxadiazole 84.1% activity remaining Leishmania infantum
6.3.1.9 GSH substrate inhibition Trypanosoma brucei brucei
6.3.1.9 additional information screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview. No inhibition by [3-[4-([6-(3,4-dimethoxyphenyl)-7-[(ethylcarbamothioyl)amino]pyrido[2,3-d]pyrimidin-2-yl]amino)phenyl]propyl]molybdenum(1+) and 1,4,7-trioxo-1lambda5,4lambda5,7lambda5-benzo[1,2-c:3,4-c':5,6-c'']tris[1,2,5]oxadiazole Leishmania infantum
6.3.1.9 additional information screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview. No effect by 8-[(2E)-2-[(2,4-dihydroxyphenyl)methylidene]hydrazinyl]-7-(2-hydroxy-3-phenoxypropyl)-1,3-dimethyl-3,7-dihydro-1H-purine-2,6-dione and J31 Trypanosoma brucei brucei
6.3.1.9 additional information screening of different scaffold-derived compounds for inhibitory activity on enzyme TryS, overview Trypanosoma cruzi
6.3.1.9 N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
-
 Leishmania infantum
6.3.1.9 N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea) 24.3% activity remaining Trypanosoma brucei brucei
6.3.1.9 N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea) 61.3% activity remaining Trypanosoma cruzi
6.3.1.9 N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
-
 Leishmania infantum
6.3.1.9 N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea) 25.3% activity remaining Trypanosoma brucei brucei
6.3.1.9 N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea) 52.2% activity remaining Trypanosoma cruzi
6.3.1.9 N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide i.e. FS-554 Leishmania infantum
6.3.1.9 N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide i.e. FS-554 Trypanosoma brucei brucei
6.3.1.9 N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide i.e. FS-554, 40.5% activity remaining Trypanosoma cruzi
6.3.1.9 N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
-
 Leishmania infantum
6.3.1.9 N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea 20.9% activity remaining Trypanosoma brucei brucei
6.3.1.9 N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea 65.4% activity remaining Trypanosoma cruzi
6.3.1.9 N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
-
 Leishmania infantum
6.3.1.9 N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea 48.7% activity remaining Trypanosoma brucei brucei
6.3.1.9 N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea 32.3% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
-
 Leishmania infantum
6.3.1.9 N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine 51.1% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine 53.5% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
-
 Leishmania infantum
6.3.1.9 N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine 47.5% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine 30.8% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
-
 Leishmania infantum
6.3.1.9 N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine 42.4% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine 23.8% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N3-bis[(4-methoxyphenyl)methyl]propane-1,3-diamine
-
 Trypanosoma cruzi
6.3.1.9 N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
-
 Leishmania infantum
6.3.1.9 N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine 50.9% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine 25.6% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine
-
 Leishmania infantum
6.3.1.9 N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine 37.9% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine 26.6% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
-
 Leishmania infantum
6.3.1.9 N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine 61.2% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine 17.0% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
-
 Leishmania infantum
6.3.1.9 N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine 48.9% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine 33.4% activity remaining Trypanosoma cruzi
6.3.1.9 N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
-
 Leishmania infantum
6.3.1.9 N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine 55.6% activity remaining Trypanosoma brucei brucei
6.3.1.9 N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine 28.9% activity remaining Trypanosoma cruzi
6.3.1.9 [3-[4-([6-(3,4-dimethoxyphenyl)-7-[(ethylcarbamothioyl)amino]pyrido[2,3-d]pyrimidin-2-yl]amino)phenyl]propyl]molybdenum(1+) 81.3% activity remaining Trypanosoma brucei brucei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.9 additional information
-
 additional information Michaelis-Menten steady-state kinetics Trypanosoma cruzi
6.3.1.9 additional information
-
 additional information Michaelis-Menten steady-state kinetics Leishmania infantum
6.3.1.9 additional information
-
 additional information Michaelis-Menten steady-state kinetics Trypanosoma brucei brucei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.9 Mg2+ required Trypanosoma cruzi
6.3.1.9 Mg2+ required Leishmania infantum
6.3.1.9 Mg2+ required Trypanosoma brucei brucei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma cruzi
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Leishmania infantum
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma brucei brucei
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma cruzi CL Brener
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Trypanosoma brucei brucei 427
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263
-
 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma cruzi
-
 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Leishmania infantum
-
 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma brucei brucei
-
 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma cruzi CL Brener
-
 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Trypanosoma brucei brucei 427
-
 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263
-
 glutathionylspermidine + ADP + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.9 Leishmania infantum A4I2Z3
-
-
6.3.1.9 Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263 A4I2Z3
-
-
6.3.1.9 Trypanosoma brucei brucei Q8IEX1 cell line 514-1313
-
6.3.1.9 Trypanosoma brucei brucei 427 Q8IEX1 cell line 514-1313
-
6.3.1.9 Trypanosoma cruzi Q9GT49
-
-
6.3.1.9 Trypanosoma cruzi CL Brener Q9GT49
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.9 recombinant His-tagged enzyme from Escherichia coli strains BL21(DE3) or Tuner (DE3) by affinity chromatography Trypanosoma cruzi
6.3.1.9 recombinant His-tagged enzyme from Escherichia coli strains BL21(DE3) or Tuner (DE3) by affinity chromatography Leishmania infantum
6.3.1.9 recombinant His-tagged enzyme from Escherichia coli strains BL21(DE3) or Tuner (DE3) by affinity chromatography Trypanosoma brucei brucei

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.1.9 promastigote
-
 Leishmania infantum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
 Trypanosoma cruzi N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
 Leishmania infantum N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
 Trypanosoma brucei brucei N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
 Trypanosoma cruzi CL Brener N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
 Trypanosoma brucei brucei 427 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + glutathionylspermidine + ATP
-
 Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263 N1,N8-bis(glutathionyl)spermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP
-
 Trypanosoma cruzi glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP
-
 Leishmania infantum glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP
-
 Trypanosoma brucei brucei glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP
-
 Trypanosoma cruzi CL Brener glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP
-
 Trypanosoma brucei brucei 427 glutathionylspermidine + ADP + phosphate
-
 ?
6.3.1.9 glutathione + spermidine + ATP
-
 Leishmania infantum JPCM5 /MHOM / MA / 67 / ITMAP263 glutathionylspermidine + ADP + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.9 LiTRYS
-
 Leishmania infantum
6.3.1.9 TbTryS
-
 Trypanosoma brucei brucei
6.3.1.9 TcTryS
-
 Trypanosoma cruzi
6.3.1.9 TRS
-
 Trypanosoma cruzi
6.3.1.9 TryS
-
 Trypanosoma cruzi
6.3.1.9 TryS
-
 Leishmania infantum
6.3.1.9 TryS
-
 Trypanosoma brucei brucei
6.3.1.9 tyrS
-
 Trypanosoma brucei brucei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.9 22
-
 assay at room temperature Trypanosoma cruzi
6.3.1.9 22
-
 assay at room temperature Leishmania infantum
6.3.1.9 22
-
 assay at room temperature Trypanosoma brucei brucei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.9 7.4
-
 assay at Trypanosoma cruzi
6.3.1.9 7.4
-
 assay at Leishmania infantum
6.3.1.9 7.4
-
 assay at Trypanosoma brucei brucei

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.9 ATP
-
 Trypanosoma cruzi
6.3.1.9 ATP
-
 Leishmania infantum
6.3.1.9 ATP
-
 Trypanosoma brucei brucei

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.1.9 0.00015
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum 2-(9-bromo-3-chloro-6-oxo-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl)-N-[2-(methylamino)ethyl]acetamide
6.3.1.9 0.00035
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N-[2-(methylamino)ethyl]-2-[6-oxo-9-(trifluoromethyl)-7,12-dihydroindolo[3,2-d][1]benzazepin-5(6H)-yl]acetamide
6.3.1.9 0.0136
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N-[2-amino-6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-ethylthiourea
6.3.1.9 0.0138
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N,N'-[6-(2,5-dichlorophenyl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
6.3.1.9 0.0153
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N,N'-[6-(2H-1,3-benzodioxol-5-yl)pyrido[2,3-d]pyrimidine-2,7-diyl]bis(N'-tert-butylurea)
6.3.1.9 0.0234
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N4-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]butane-1,4-diamine
6.3.1.9 0.0259
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N12-bis[(4-bromophenyl)methyl]dodecane-1,12-diamine
6.3.1.9 0.0263
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N6-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]hexane-1,6-diamine
6.3.1.9 0.0313
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum 5-oxo-1H,3H-5lambda5-benzo[1,2-c:4,5-c']bis[1,2,5]oxadiazole
6.3.1.9 0.0321
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N-[2-amino-6-(naphthalen-2-yl)pyrido[2,3-d]pyrimidin-7-yl]-N'-tert-butylurea
6.3.1.9 0.0391
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N6-bis[[4-(benzyloxy)phenyl]methyl]hexane-1,6-diamine
6.3.1.9 0.0478
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N10-bis[[4-(propan-2-yl)phenyl]methyl]decane-1,10-diamine
6.3.1.9 0.0496
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N8-bis[[4-(benzyloxy)-3-methoxyphenyl]methyl]octane-1,8-diamine
6.3.1.9 0.0515
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N12-bis[(4-chlorophenyl)methyl]dodecane-1,12-diamine
6.3.1.9 0.0567
-
 pH 7.4, 22°C, recombinant enzyme Leishmania infantum N1,N4-bis[[4-(benzyloxy)phenyl]methyl]butane-1,4-diamine

General Information

EC Number General Information Comment Organism
6.3.1.9 metabolism trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase Trypanosoma cruzi
6.3.1.9 metabolism trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase Leishmania infantum
6.3.1.9 metabolism trypanothione dependent redox metabolism, overview. Trypanothione synthetase catalyzes the ligation of two molecules of gluthatione to one of spermidine using the energy provided by two ATP molecules. Trypanothione reductase maintains trypanothione in the reduced state at expenses of NADPH, which can be supplied by the oxidative phase of the pentose phosphate pathway via glucose 6-phosphate dehydrogenase Trypanosoma brucei brucei
6.3.1.9 physiological function in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis Trypanosoma cruzi
6.3.1.9 physiological function in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis Leishmania infantum
6.3.1.9 physiological function in pathogenic trypanosomatids, a single enzyme, trypanothione synthetase (TryS), catalyzes trypanothione biosynthesis, which is indispensable for parasite survival. Reduced trypanothione is involved in multiple functions such as the detoxification of xenobiotics, cell proliferation, defense against oxidants and protein thiol-redox homeostasis Trypanosoma brucei brucei