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Literature summary extracted from
- Abscisic acid metabolic genes of wheat (Triticum aestivum L.) identification and insights into their functionality in seed dormancy and dehydration tolerance (2016), Planta, 244, 429-447 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.13.11.51 | gene NCED2, DNA and amino acid sequence determination and analysis, cloning from seedlings, sequence comparisons and phylogenetic analysis, expression analysis, quantitative and semiquantitative real-time and RT-PCR expression analysis | Triticum aestivum |
| 1.13.11.51 | gene NCED2A, DNA and amino acid sequence determination and analysis, cloning from seedlings, sequence comparisons and phylogenetic analysis, expression analysis, quantitative and semiquantitative real-time and RT-PCR expression analysis, recombinant expressionin tramsgemic Arabidopsis thaliana plants using the transformtaion method with Agrobacterium tumefaciens strain AGL1 harboring the CaMV35S::TaNCED2A construct | Triticum aestivum |
| 1.13.11.51 | gene NCED2B, DNA and amino acid sequence determination and analysis, cloning from seedlings, sequence comparisons and phylogenetic analysis, expression analysis, quantitative and semiquantitative real-time and RT-PCR expression analysis | Triticum aestivum |
| 1.13.11.51 | gene NCED2D, DNA and amino acid sequence determination and analysis, cloning from seedlings, sequence comparisons and phylogenetic analysis, expression analysis, quantitative and semiquantitative real-time and RT-PCR expression analysis | Triticum aestivum |
| 1.14.14.137 | gene TaCYP707A1B, cloning from seedlings, expression analysis, ectopic expression of genes TaNCED2A and TaCYP707A1B in Arabidopsis thaliana Col-0 ecotype, using Agrobacterium tumefaciens AGL1 transformation method, results in altered seed abscisic acid level and dormancy with no effect on leaf abscisic acid content and transpirational water loss, phenotypes, overview. Quantitative and semiquantitative real-time and RT-PCR expression analysis | Triticum aestivum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.137 | additional information | generation of Tricticum aestivum mutant lines cyp707a1, cyp707a2, and cyp707a1/cyp707a2, phenotypes, overview | Triticum aestivum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.51 | a 9-cis-epoxycarotenoid + O2 | Triticum aestivum | - |
2-cis,4-trans-xanthoxin + a 12'-apo-carotenal | - |
? |  |
| 1.14.14.137 | (+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | Triticum aestivum | - |
8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.13.11.51 | Triticum aestivum | - |
cv. AC Domain | - |
| 1.13.11.51 | Triticum aestivum | A0A0M4UGS6 | NCED2; cv. AC Domain | - |
| 1.13.11.51 | Triticum aestivum | A0A1B3B582 | cv. AC Domain | - |
| 1.13.11.51 | Triticum aestivum | A0A1B3B5A6 | cv. AC Domain | - |
| 1.14.14.137 | Triticum aestivum | B8QBY2 | cv. AC Domain | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.13.11.51 | additional information | tissue-specific expression pattern analysis of NCEDs isozymes | Triticum aestivum | - |
| 1.13.11.51 | additional information | tissue-specific expression pattern analysis of NCEDs isozymes, comparision of expression of TaNCED2 and TaCYP707A1 in dormant and non-dormant wheat seeds | Triticum aestivum | - |
| 1.13.11.51 | additional information | tissue-specific expression pattern analysis of NCEDs isozymes, spatiotemporal expression of TaNCED2, overview. Comparision of expression of TaNCED2 and TaCYP707A1 in dormant and non-dormant wheat seeds | Triticum aestivum | - |
| 1.13.11.51 | seed | - |
Triticum aestivum | - |
| 1.13.11.51 | seedling | two-weeks-old | Triticum aestivum | - |
| 1.13.11.51 | silique | - |
Triticum aestivum | - |
| 1.14.14.137 | seedling | spatiotemporal expression pattern of TaCYP707A1B gene in hexaploid wheat | Triticum aestivum | - |
| 1.14.14.137 | silique | CYP707A1 transcripts are highly abundant in the silique | Triticum aestivum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.13.11.51 | a 9-cis-epoxycarotenoid + O2 | - |
Triticum aestivum | 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal | - |
? | |
| 1.14.14.137 | (+)-abscisate + [reduced NADPH-hemoprotein reductase] + O2 | - |
Triticum aestivum | 8'-hydroxyabscisate + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.13.11.51 | 9-cis-epoxycarotenoid dioxygenase | - |
Triticum aestivum |
| 1.13.11.51 | NCED | - |
Triticum aestivum |
| 1.13.11.51 | NCED2 | - |
Triticum aestivum |
| 1.13.11.51 | NCED2A | - |
Triticum aestivum |
| 1.13.11.51 | NCED2B | - |
Triticum aestivum |
| 1.13.11.51 | NCED2D | - |
Triticum aestivum |
| 1.14.14.137 | ABA 8'-hydroxylase | - |
Triticum aestivum |
| 1.14.14.137 | ABA8'OH | - |
Triticum aestivum |
| 1.14.14.137 | CYP707A1 | - |
Triticum aestivum |
| 1.14.14.137 | cytochrome P450 monooxygenase 707A1 | - |
Triticum aestivum |
| 1.14.14.137 | TaABA8'OH1 | - |
Triticum aestivum |
| 1.14.14.137 | TaCYP707A1B | - |
Triticum aestivum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.137 | cytochrome P-450 | - |
Triticum aestivum | |
| 1.14.14.137 | NADPH-hemoprotein reductase | A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ | Triticum aestivum |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.13.11.51 | malfunction | similar rate of transpirational water loss between the wild-type and mutant TaNCED2A-OE, cyp707a1/cyp707a2 and TaCYP707A1B-C lines during the entire period of dehydration | Triticum aestivum |
| 1.13.11.51 | metabolism | biosynthesis and catabolism of abscisic acid (ABA) in plants are primarily regulated by 9-cis-epoxycarotenoid dioxygenases (NCEDs) and ABA 8'-hydroxylase (ABA8'OH, EC 1.14.13.93), respectively | Triticum aestivum |
| 1.13.11.51 | physiological function | role of NCEDs in regulating plant response to environmental stresses | Triticum aestivum |
| 1.14.14.137 | evolution | the enzyme is a member of the CYP707A family | Triticum aestivum |
| 1.14.14.137 | malfunction | deletion in TaABA8'OH1 leads to increased abscisic acid level in the spikes and therefore increased drought sensitivity, phenotype, overview. Germination of the cyp707a1 cyp707a2 seeds appear not to be affected by the cold treatment, which induces early germination phenotype in the control wild-type and TaCYP707A1B-C seeds of transgenic Arabidospis thaliana, similar rate of transpirational water loss between the wild-type and mutant TaNCED2A-OE, cyp707a1/cyp707a2 and TaCYP707A1B-C lines during the entire period of dehydration | Triticum aestivum |
| 1.14.14.137 | metabolism | biosynthesis and catabolism of abscisic acid (ABA) in plants are primarily regulated by 9-cis-epoxycarotenoid dioxygenases (NCEDs) and ABA 8'-hydroxylase (ABA8'OH), respectively | Triticum aestivum |
| 1.14.14.137 | physiological function | the abscisic acid catabolic enzyme ABA8'OH is encoded by members of the cytochrome P450 monooxygenase 707A (CYP707A) gene family, which play important roles in regulating seed abscisic acid level during seed development and therefore dormancy. Role of the B genome copy of the cytochrome P450 monooxygenase 707A1 (CYP707A1) gene of hexaploid wheat (TaCYP707A1B), which encodes ABA8'OH, in regulating seed dormancy and leaf dehydration tolerance | Triticum aestivum |
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