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Literature summary extracted from

  • Schwinn, K.; Miosic, S.; Davies, K.; Thill, J.; Gotame, T.P.; Stich, K.; Halbwirth, H.
    The B-ring hydroxylation pattern of anthocyanins can be determined through activity of the flavonoid 3-hydroxylase on leucoanthocyanidins (2014), Planta, 240, 1003-1010 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.14.81 0.001
-
 kaempferol pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 0.001
-
 naringenin pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 0.0058
-
 apigenin pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 0.009
-
 dihydrokaempferol pH 7.0, 25°C Billardiera heterophylla
1.14.14.82 0.001
-
 kaempferol pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.001
-
 naringenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.0012
-
 naringenin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 0.0013
-
 kaempferol pH 7.0, 25°C Tagetes erecta
1.14.14.82 0.0019
-
 kaempferol pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 0.002
-
 dihydrokaempferol pH 7.0, 25°C Tagetes erecta
1.14.14.82 0.003
-
 naringenin pH 7.0, 25°C Tagetes erecta
1.14.14.82 0.0054
-
 apigenin pH 7.0, 25°C Tagetes erecta
1.14.14.82 0.0058
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.0064
-
 apigenin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 0.0074
-
 5-deoxyleucopelargonidin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 0.0079
-
 5-deoxyleucopelargonidin pH 7.0, 25°C Tagetes erecta
1.14.14.82 0.009
-
 dihydrokaempferol pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 0.009
-
 dihydrokaempferol pH 7.3, 25°C Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.81 Billardiera heterophylla U3LXJ8
-
-
1.14.14.82 Arabidopsis thaliana Q9SD85
-
-
1.14.14.82 Fragaria x ananassa
-
-
-
1.14.14.82 Tagetes erecta C1J0N9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.81 5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Billardiera heterophylla ? + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Billardiera heterophylla tricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 dihydrokaempferol + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Billardiera heterophylla dihydromyricetin + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.81 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Billardiera heterophylla myricetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.81 naringenin + 2 [reduced NADPH-hemoprotein reductase] + 2 O2
-
 Billardiera heterophylla pentahydroxyflavanone + 2 [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.82 5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana 5-deoxyleucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 5-deoxyleucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta 5-deoxyleucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 apigenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta luteolin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 dihydrokaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta dihydroquercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 kaempferol + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta quercetin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 leucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa leucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 leucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana leucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 leucopelargonidin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta leucocyanidin + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Fragaria x ananassa eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Arabidopsis thaliana eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.82 naringenin + [reduced NADPH-hemoprotein reductase] + O2
-
 Tagetes erecta eriodictyol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.14.81 10.6
-
 naringenin pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 13.4
-
 apigenin pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 17
-
 kaempferol pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 81.4
-
 dihydrokaempferol pH 7.0, 25°C Billardiera heterophylla
1.14.14.82 2.1
-
 naringenin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 5.8
-
 kaempferol pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 9.3
-
 apigenin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 10.2
-
 kaempferol pH 7.0, 25°C Tagetes erecta
1.14.14.82 10.6
-
 naringenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 12.3
-
 5-deoxyleucopelargonidin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 12.5
-
 dihydrokaempferol pH 7.0, 25°C Tagetes erecta
1.14.14.82 12.7
-
 dihydrokaempferol pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 13.4
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 17
-
 kaempferol pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 17
-
 naringenin pH 7.0, 25°C Tagetes erecta
1.14.14.82 22.1
-
 apigenin pH 7.0, 25°C Tagetes erecta
1.14.14.82 38.7
-
 5-deoxyleucopelargonidin pH 7.0, 25°C Tagetes erecta
1.14.14.82 81.4
-
 dihydrokaempferol pH 7.0, 25°C Fragaria x ananassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.81 7.3
-
-
 Billardiera heterophylla
1.14.14.82 7.3
-
-
 Fragaria x ananassa
1.14.14.82 7.3
-
-
 Arabidopsis thaliana
1.14.14.82 7.3
-
-
 Tagetes erecta

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.14.81 900
-
 dihydrokaempferol pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 1700
-
 kaempferol pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 2300
-
 apigenin pH 7.0, 25°C Billardiera heterophylla
1.14.14.81 10000
-
 naringenin pH 7.0, 25°C Billardiera heterophylla
1.14.14.82 900
-
 dihydrokaempferol pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 1400
-
 dihydrokaempferol pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 1500
-
 apigenin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 1700
-
 kaempferol pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 1700
-
 5-deoxyleucopelargonidin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 1800
-
 naringenin pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 2300
-
 apigenin pH 7.0, 25°C Fragaria x ananassa
1.14.14.82 3100
-
 kaempferol pH 7.3, 25°C Arabidopsis thaliana
1.14.14.82 4100
-
 apigenin pH 7.0, 25°C Tagetes erecta
1.14.14.82 5000
-
 5-deoxyleucopelargonidin pH 7.0, 25°C Tagetes erecta
1.14.14.82 5600
-
 naringenin pH 7.0, 25°C Tagetes erecta
1.14.14.82 6300
-
 dihydrokaempferol pH 7.0, 25°C Tagetes erecta
1.14.14.82 7700
-
 kaempferol pH 7.0, 25°C Tagetes erecta
1.14.14.82 10000
-
 naringenin pH 7.0, 25°C Fragaria x ananassa