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Literature summary extracted from

  • Suza, W.; Staswick, P.
    The role of JAR1 in jasmonoyl-L-isoleucine production during Arabidopsis wound response (2008), Planta, 227, 1221-1232 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.52 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.52 0.03
-
L-Ile at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 0.19
-
(-)-jasmonate at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 1.84
-
L-Phe at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 1.93
-
L-Leu at pH 8.6 and 25°C Arabidopsis thaliana
6.3.2.52 2.49
-
L-Val at pH 8.6 and 25°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.52 ATP + (-)-jasmonate + L-Ile Arabidopsis thaliana L-Ile is the preferred amino acid AMP + diphosphate + jasmonoyl-L-Ile
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.52 Arabidopsis thaliana
-
ecotype Col-0
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.52 flower
-
Arabidopsis thaliana
-
6.3.2.52 leaf
-
Arabidopsis thaliana
-
6.3.2.52 root
-
Arabidopsis thaliana
-
6.3.2.52 seedling
-
Arabidopsis thaliana
-
6.3.2.52 shoot
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.52 ATP + (-)-jasmonate + L-Ile L-Ile is the preferred amino acid Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Ile
-
?
6.3.2.52 ATP + (-)-jasmonate + L-Leu
-
Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Leu
-
?
6.3.2.52 ATP + (-)-jasmonate + L-Phe
-
Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Phe
-
?
6.3.2.52 ATP + (-)-jasmonate + L-Val
-
Arabidopsis thaliana AMP + diphosphate + jasmonoyl-L-Val
-
?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.52 JAR1
-
Arabidopsis thaliana
6.3.2.52 jasmonate: amino acid synthetase
-
Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
6.3.2.52 Arabidopsis thaliana the enzyme transcript increases dramatically in wounded tissue, reaching a maximum after about 1 h up