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Literature summary extracted from

  • Kai, K.; Kasa, S.; Sakamoto, M.; Aoki, N.; Watabe, G.; Yuasa, T.; Iwaya-Inoue, M.; Ishibashi, Y.
    Role of reactive oxygen species produced by NADPH oxidase in gibberellin biosynthesis during barley seed germination (2016), Plant Signal. Behav., 11, e1180492 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.107 gene HvKAO1, quantitative RT-PCR enzyme expression analysis Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.107 ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 Hordeum vulgare overall reaction gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.107 Hordeum vulgare Q9AXH9 cv. Himalaya
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.107 embryo
-
 Hordeum vulgare
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1.14.14.107 seed
-
 Hordeum vulgare
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.107 ent-kaur-16-en-19-oate + 3 [reduced NADPH-hemoprotein reductase] + 3 O2 overall reaction Hordeum vulgare gibberellin A12 + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.107 ent-kaurenoic acid oxidase 1 UniProt Hordeum vulgare
1.14.14.107 HvKAO1
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 Hordeum vulgare
1.14.14.107 KAO
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 Hordeum vulgare

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.107 cytochrome P-450
-
 Hordeum vulgare
1.14.14.107 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Hordeum vulgare

Expression

EC Number Organism Comment Expression
1.14.14.107 Hordeum vulgare diphenyleneiodonium reduces the expression of HvKAO1, about 20% at 1 mM down
1.14.14.107 Hordeum vulgare exogenous H2O2 promotes the expression of HvKAO1 in barley embryos up

General Information

EC Number General Information Comment Organism
1.14.14.107 physiological function reactive oxygen species (ROS), produced by NADPH oxidase, are involved in gibberellin biosynthesis through the regulation of ent-kaurenoic acid oxidase HvKAO1 and GA3-oxidase HvGA3ox1, relationship between ROS and gibberellin (GA) biosynthesis enzymes, overview Hordeum vulgare