BRENDA - Enzyme Database

Molecular cloning and identification of a flavanone 3-hydroxylase gene from Lycium chinense, and its overexpression enhances drought stress in tobacco

Song, X.; Diao, J.; Ji, J.; Wang, G.; Guan, C.; Jin, C.; Wang, Y.; Plant Physiol. Biochem. 98, 89-100 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene LcF3H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3), recombinant overexpression in leaves of Nicotiana tabacum cv. Xanthinc via transformation with Agrobacterium tumefaciens strain EHA105, overexpression of heterogenous LcF3H enhances the expression levels of downstream genes but does not cause alteration in the expression of upstream genes including endogenous F3H of Nicotiana tabacum. The enzyme's overexpression does cause a significant increase in the content of three major flavan-3-ols, catechin, epicatechin and epigallocatechin
Lycium chinense
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Lycium chinense
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Lycium chinense
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Lycium chinense
A0A068EM79
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
flower
-
Lycium chinense
-
1.14.11.9
leaf
primordial leaf, young leaf (highest level), and mature leaf
Lycium chinense
-
1.14.11.9
additional information
tissue pattern of enzyme expression, overview
Lycium chinense
-
1.14.11.9
root
low expression level
Lycium chinense
-
1.14.11.9
seedling
-
Lycium chinense
-
1.14.11.9
stem
-
Lycium chinense
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746138
Lycium chinense
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746138
Lycium chinense
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
flavanone 3-hydroxylase
-
Lycium chinense
1.14.11.9
LcF3H
-
Lycium chinense
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene LcF3H, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative PCR enzyme expression analysis, recombinant expression in Escherichia coli strain BL21(DE3), recombinant overexpression in leaves of Nicotiana tabacum cv. Xanthinc via transformation with Agrobacterium tumefaciens strain EHA105, overexpression of heterogenous LcF3H enhances the expression levels of downstream genes but does not cause alteration in the expression of upstream genes including endogenous F3H of Nicotiana tabacum. The enzyme's overexpression does cause a significant increase in the content of three major flavan-3-ols, catechin, epicatechin and epigallocatechin
Lycium chinense
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Lycium chinense
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Lycium chinense
-
dihydrokaempferol + succinate + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
flower
-
Lycium chinense
-
1.14.11.9
leaf
primordial leaf, young leaf (highest level), and mature leaf
Lycium chinense
-
1.14.11.9
additional information
tissue pattern of enzyme expression, overview
Lycium chinense
-
1.14.11.9
root
low expression level
Lycium chinense
-
1.14.11.9
seedling
-
Lycium chinense
-
1.14.11.9
stem
-
Lycium chinense
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746138
Lycium chinense
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746138
Lycium chinense
dihydrokaempferol + succinate + CO2
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.14.11.9
Lycium chinense
the transcript levels of LcF3H shows significant decrease when plants are treated by exogenous nordihydroguaiaretic acid (100 mM) application together with drought stress treatment comparing to that treated with drought stress alone, the decrease in LcF3H expression under drought stress is less in overexpressing lines compared to the control plants
down
1.14.11.9
Lycium chinense
positive correlation between absisic acid and LcF3H expression level
additional information
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
metabolism
positive correlation between absisic acid and LcF3H expression level
Lycium chinense
1.14.11.9
physiological function
the flavanone 3-hydroxylase (F3H) gene which encodes flavanone 3-hydroxylase, is essential in flavonoids biosynthetic pathway. Lycium chinense (L. chinense) is a deciduous woody perennial halophyte that grows under a large variety of environmental conditions and survives under extreme drought stress, possible relationship between the oxidative damage and the regulation of LcF3H gene expression in Lycium chinense under drought stress
Lycium chinense
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
metabolism
positive correlation between absisic acid and LcF3H expression level
Lycium chinense
1.14.11.9
physiological function
the flavanone 3-hydroxylase (F3H) gene which encodes flavanone 3-hydroxylase, is essential in flavonoids biosynthetic pathway. Lycium chinense (L. chinense) is a deciduous woody perennial halophyte that grows under a large variety of environmental conditions and survives under extreme drought stress, possible relationship between the oxidative damage and the regulation of LcF3H gene expression in Lycium chinense under drought stress
Lycium chinense
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.11.9
Lycium chinense
the transcript levels of LcF3H shows significant decrease when plants are treated by exogenous nordihydroguaiaretic acid (100 mM) application together with drought stress treatment comparing to that treated with drought stress alone, the decrease in LcF3H expression under drought stress is less in overexpressing lines compared to the control plants
down
1.14.11.9
Lycium chinense
positive correlation between absisic acid and LcF3H expression level
additional information