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Literature summary extracted from
- RNA interference of the nicotine demethylase gene CYP82E4v1 reduces nornicotine content and enhances Myzus persicae resistance in Nicotiana tabacum L (2016), Plant Physiol. Biochem., 107, 214-221 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.14.B11 | gene CYP82E4v1, DNA and amino acid sequence determination and analysis, real-time PCR enzyme expression analysis | Nicotiana tabacum |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.14.B11 | additional information | enzyme silencing by CYP82E21-specific RNAi expression. CYP82E4v1-interference affects the accumulation of nicotine and nornicotine, and the RNAi vector for CYP82E4v1 effectively silences CYP82E4v1 expression and further blocks the synthesis of nornicotine | Nicotiana tabacum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.B11 | nicotine + NADPH + H+ | Nicotiana tabacum | - |
nornicotine + NADP+ | - |
? |  |
| 1.14.14.B11 | nicotine + [reduced NADPH-hemoprotein reductase] + O2 | Nicotiana tabacum | - |
nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.B11 | Nicotiana tabacum | Q38Q86 | var. K326 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.B11 | leaf | - |
Nicotiana tabacum | - |
| 1.14.14.B11 | root | highest expression level of the enzyme | Nicotiana tabacum | - |
| 1.14.14.B11 | stem | - |
Nicotiana tabacum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.B11 | nicotine + NADPH + H+ | - |
Nicotiana tabacum | nornicotine + NADP+ | - |
? | |
| 1.14.14.B11 | nicotine + [reduced NADPH-hemoprotein reductase] + O2 | - |
Nicotiana tabacum | nornicotine + formaldehyde + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.B11 | CYP82E4v1 | - |
Nicotiana tabacum |
| 1.14.14.B11 | nicotine N-demethylase | - |
Nicotiana tabacum |
| 1.14.14.B11 | NND | - |
Nicotiana tabacum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.B11 | cytochrome P450 | - |
Nicotiana tabacum | |
| 1.14.14.B11 | NADPH | - |
Nicotiana tabacum | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.14.B11 | malfunction | RNA interference of the nicotine demethylase gene CYP82E4v1 reduces nornicotine content and enhances Myzus persicae resistance in Nicotiana tabacum infected with aphids | Nicotiana tabacum |
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