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Literature summary extracted from

  • Dutta, A.; Choudhary, P.; Caruana, J.; Raina, R.
    JMJ27, an Arabidopsis H3K9 histone demethylase, modulates defense against Pseudomonas syringae and flowering time (2017), Plant J., 91, 1015-1028 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.65 gene At4g00990, quantitative and nonquantitative reverse transcription PCR enzyme expression analysis, construction of transgenic plants expressing a JMJ27 promoter-GUS fusion, A sequence containing approximately 1850 bp immediately upstream of the JMJ27 translation start site is fused to the beta-glucuronidase (GUS) reporter gene, and the construct is transformed into wild-type plants. GUS analysis is carried out at various stages of plant growth and in different organs. JMJ27 promoter activity is detected in 7-day-old seedlings, flowers and siliques. A reporter construct containing JMJ27 cDNA fused in-frame to GFP cDNA is and expressed from the constitutive 35S promoter (35S::JMJ27-GFP) in onion epidermal cells in the nucleus. Recombinant enzyme expression as JMJ27-MBP fusion protein Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.65 additional information construction of two T-DNA insertion lines, jmj27-1 (SALK_092672) and jmj27-2 (SALK_131899), these mutants are null alleles. No visible phenotypic differences are observed in the mutant lines as compared with the wild-type Col-0, except that the mutants flowered earlier than the wild-type plants. Lesion mimic mutants (dll1, acd2, hrl1, lsd1) constitutively express several defense-related genes at high levels, and display enhanced resistance against a variety of pathogens Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.65 nucleus JMJ27 is a nuclear protein containing a zinc-finger motif Arabidopsis thaliana 5634
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.65 Fe2+ JMJ27 is a nuclear protein containing a zinc-finger motif and a catalytic JmjC domain with conserved Fe(II) and alpha-ketoglutarate binding sites Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.65 histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2 Arabidopsis thaliana
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histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
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?
1.14.11.65 additional information Arabidopsis thaliana JMJ27 displays H3K9me1/2 demethylase activity both in vitro and in vivo ?
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?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.65 Arabidopsis thaliana Q8VYB9
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.65 flower
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Arabidopsis thaliana
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1.14.11.65 additional information analysis of temporal and spatial expression of JMJ27 Arabidopsis thaliana
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1.14.11.65 seedling
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Arabidopsis thaliana
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1.14.11.65 silique
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Arabidopsis thaliana
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.65 histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2
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Arabidopsis thaliana histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
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?
1.14.11.65 histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2 calf thymus histone substrate Arabidopsis thaliana histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
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?
1.14.11.65 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 calf thymus histone substrate Arabidopsis thaliana histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
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?
1.14.11.65 additional information JMJ27 displays H3K9me1/2 demethylase activity both in vitro and in vivo Arabidopsis thaliana ?
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1.14.11.65 additional information enzyme substrate specificity for different lysine residues on different histones, JMJ27 is specific for lysine 9 residues of histone H3, overview Arabidopsis thaliana ?
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?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.65 AtJMJ17
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Arabidopsis thaliana
1.14.11.65 H3K9 histone demethylase
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Arabidopsis thaliana
1.14.11.65 JMJ27
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Arabidopsis thaliana
1.14.11.65 JmjC domain-containing protein 27
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Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.65 37
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assay at Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.11.65 additional information
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JMJ27 is a heat-labile enzyme Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
1.14.11.65 Arabidopsis thaliana JMJ27 is induced in response to virulent Pseudomonas syringae pathogens, i.e. Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) up

General Information

EC Number General Information Comment Organism
1.14.11.65 evolution the enzyme belongs to the JHDM2 (JmjC domain-containing histone demethylase 2) family of enzymes which modulates defense against pathogens and flowering time Arabidopsis thaliana
1.14.11.65 malfunction loss of JMJ27 function leads to early flowering. Expression of PR genes is compromised in jmj27 mutants. Loss of JMJ27 function leads to hypermethylation of histones (H3) at PR1 and WRKY25 promoters, mutant phenotypes, overview. No significant difference is observed at the FT locus in jmj27 mutants compared with the wild-type, indicating that JMJ27 does not modulate H3K9me2 levels at this locus Arabidopsis thaliana
1.14.11.65 physiological function JMJ27 displays H3K9me1/2 demethylase activity both in vitro and in vivo. JMJ27 is induced in response to virulent Pseudomonas syringae pathogens and is required for resistance against these pathogens. JMJ27 is a negative modulator of WRKY25 (a repressor of defense) and a positive modulator of several pathogenesis-related (PR) proteins. It negatively modulates the major flowering regulator Constans (CO) and positively modulates Flowering locus C (FLC). JMJ27 functions as a histone demethylase to modulate both physiological (defense) and developmental (flowering time) processes in Arabidopsis. JMJ27 negatively modulates expression of WRKY genes. JMJ27 modulates flowering time by regulating expression of major flowering time genes through modulating histone methylation Arabidopsis thaliana