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Literature summary extracted from

  • Lee, J.; Joshi, N.; Pasini, R.; Dobson, R.C.; Allison, J.; Leustek, T.
    Inhibition of Arabidopsis growth by the allelopathic compound azetidine-2-carboxylate is due to the low amino acid specificity of cytosolic prolyl-tRNA synthetase (2016), Plant J., 88, 236-246 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.15 expressed in Escherichia coli BL21-Codon Plus (DE3) cells Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.15 azetidine-2-carboxylic acid at 0.2 mM isozyme ProRS-Org is inhibited by 25% Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.15 0.06
-
 L-proline isozyme ProRS-Cyt, at pH 7.2 and 30°C Arabidopsis thaliana
6.1.1.15 0.1
-
 L-proline isozyme ProRS-Org, at pH 7.2 and 30°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.15 chloroplast isozyme ProRS-Org Arabidopsis thaliana 9507
-
6.1.1.15 cytosol isozyme ProRS-Cyt Arabidopsis thaliana 5829
-
6.1.1.15 mitochondrion isozyme ProRS-Org Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro Arabidopsis thaliana
-
 AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.15 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.15 Ni-NTA column chromatography Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.15 callus
-
 Arabidopsis thaliana
-
6.1.1.15 cotyledon
-
 Arabidopsis thaliana
-
6.1.1.15 root
-
 Arabidopsis thaliana
-
6.1.1.15 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.15 ATP + L-proline + tRNAPro
-
 Arabidopsis thaliana AMP + diphosphate + L-prolyl-tRNAPro
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.15 Prolyl-tRNA synthetase
-
 Arabidopsis thaliana
6.1.1.15 ProRS-Cyt isozyme Arabidopsis thaliana
6.1.1.15 ProRS-Org isozyme Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.15 13.2
-
 L-proline isozyme ProRS-Org, at pH 7.2 and 30°C Arabidopsis thaliana
6.1.1.15 57.5
-
 L-proline isozyme ProRS-Cyt, at pH 7.2 and 30°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.15 ATP
-
 Arabidopsis thaliana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.1.1.15 0.1
-
 isozyme ProRS-Org, at pH 7.2 and 30°C Arabidopsis thaliana azetidine-2-carboxylic acid

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.1.1.15 132
-
 L-proline isozyme ProRS-Org, at pH 7.2 and 30°C Arabidopsis thaliana
6.1.1.15 958
-
 L-proline isozyme ProRS-Cyt, at pH 7.2 and 30°C Arabidopsis thaliana