EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.168 | gene YUC2, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Arabidopsis thaliana |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.168 | 2-chlorophenylboronic acid | moderate inhibition of enzyme activity and primary root elongation. Probably unspecific inhibition | Arabidopsis thaliana | |
1.14.13.168 | 3,5-dibromophenylboronic acid | probably unspecific inhibition, inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
1.14.13.168 | 3,5-dichlorophenylboronic acid | probably unspecific inhibition, inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
1.14.13.168 | 3-chlorophenylboronic acid | probably unspecific inhibition, strongly inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
1.14.13.168 | 4-biphenylboronic acid | BBo, competitive, inhibits the activity of recombinant YUC in vitro, reduces endogenous indole-3-acetic acid content, and strongly inhibits primary root elongation and lateral root formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
1.14.13.168 | 4-chlorophenylboronic acid | probably unspecific inhibition, strongly inhibits root hair formation in wild-type Arabidopsis thaliana seedlings | Arabidopsis thaliana | |
1.14.13.168 | 4-methyl-4-biphenylboronic acid | weak inhibition of enzyme activity, but strong inhibition of primary root elongation | Arabidopsis thaliana | |
1.14.13.168 | 4-phenoxyphenylboronic acid | PPBo, competitive, inhibits the activity of recombinant YUC in vitro, reduces endogenous indole-3-acetic acid content, and strongly inhibits primary root elongation and lateral root formation in wild-type Arabidopsis thaliana seedlings. The compound does not interfere with the auxin response of auxin-marker genes when it was co-treated with indole acetic acid, suggesting that PPBo is not an inhibitor of auxin sensing or signaling | Arabidopsis thaliana | |
1.14.13.168 | additional information | FAD and boronic acid is not the main reason for inhibition of enzyme activity by boronic acid derivatives. Poor inhibition by 2,6-dichlorophenylboronic acid | Arabidopsis thaliana | |
1.14.13.168 | phenethylboronic acid | moderate inhibition of enzyme activity and primary root elongation | Arabidopsis thaliana | |
1.14.13.168 | phenylboronic acid | PBo, inhibition of enzyme activity, but not of primary root elongation | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.168 | (indol-3-yl)pyruvate + NADPH + H+ + O2 | Arabidopsis thaliana | - |
(indol-3-yl)acetate + NADP+ + H2O + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.168 | Arabidopsis thaliana | Q9SVQ1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.168 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.13.168 | seedling | - |
Arabidopsis thaliana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.168 | (indol-3-yl)pyruvate + NADPH + H+ + O2 | - |
Arabidopsis thaliana | (indol-3-yl)acetate + NADP+ + H2O + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.168 | flavin-containing monooxygenase YUC | - |
Arabidopsis thaliana |
1.14.13.168 | YUC2 | - |
Arabidopsis thaliana |
1.14.13.168 | YUCCA | - |
Arabidopsis thaliana |
1.14.13.168 | YUCCA2 | - |
Arabidopsis thaliana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.168 | 35 | - |
assay at | Arabidopsis thaliana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.168 | 8.5 | - |
assay at | Arabidopsis thaliana |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.168 | FAD | - |
Arabidopsis thaliana | |
1.14.13.168 | NADPH | - |
Arabidopsis thaliana |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.168 | 0.000056 | - |
4-phenoxyphenylboronic acid | pH 8.5, 35°C, recombinant His-tagged enzyme | Arabidopsis thaliana | |
1.14.13.168 | 0.000067 | - |
4-biphenylboronic acid | pH 8.5, 35°C, recombinant His-tagged enzyme | Arabidopsis thaliana |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.168 | metabolism | YUCCA, a flavin-containing monooxygenase (YUC), catalyzes the last step of conversion from indole-3-pyruvate to indole-3-acetic acid | Arabidopsis thaliana |
1.14.13.168 | physiological function | the enzyme catalzes the last step in the indole-3-pyruvate pathway, a major biosynthesis pathway in Arabidopsis thaliana for indole-3-acetic acid, the most common member of the auxin family | Arabidopsis thaliana |