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Literature summary extracted from

  • Li, C.; Liu, S.; Yao, X.; Wang, J.; Wang, T.; Zhang, Z.; Zhang, P.; Chen, K.
    PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis (2017), Plant Growth Regul., 83, 489-500 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.9 ascorbate required Pohlia nutans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.9 gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana Pohlia nutans

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.9 additional information construction of transgenic Arabidospis thaliana plants expressing the enzyme from Pohlia nutans, the Agrobacterium tumefaciens-mediated transformation of gene PnF3H confers tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, naringenin-feeding experiments and flavonoid metabolism profiling by HPLC analysis, phenotype, overview Pohlia nutans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.9 Fe2+ required for activity Pohlia nutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2 Pohlia nutans
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
?
1.14.11.9 naringenin + 2-oxoglutarate + O2 Pohlia nutans
-
dihydrokaempferol + succinate + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.9 Pohlia nutans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.9 a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
Pohlia nutans a (2R,3R)-dihydroflavonol + succinate + CO2
-
?
1.14.11.9 naringenin + 2-oxoglutarate + O2
-
Pohlia nutans dihydrokaempferol + succinate + CO2
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.9 F3H
-
Pohlia nutans
1.14.11.9 flavanone 3-hydroxylase
-
Pohlia nutans
1.14.11.9 PnF3H
-
Pohlia nutans

Expression

EC Number Organism Comment Expression
1.14.11.9 Pohlia nutans PnF3H expression levels increase by 2.49fold at 1 h and then slightly decrease to 1.47fold at 24 h with cold treatment. For the simulated drought stress (i.e. 20% PEG 6000), the PnF3H expression levels enhance by 2.39fold at 3 h and the transcript abundance remains high up to 12 h. In the UV-B radiation, the PnF3H expression levels increase and reach the maximum level of 4.57fold at 3 h. After exogenous abscisic acid treatment, the PnF3H expression levels increase quickly and reach the maximum level of 2.57fold at 0.5 h up

General Information

EC Number General Information Comment Organism
1.14.11.9 physiological function the enzyme plays a role in plant salt stress resistance. PnF3H transcripts are induced by various stress and may play a positive role in stress tolerance Pohlia nutans