PnF3H, a flavanone 3-hydroxylase from the Antarctic moss Pohlia nutans, confers tolerance to salt stress and ABA treatment in transgenic Arabidopsis
Li, C.; Liu, S.; Yao, X.; Wang, J.; Wang, T.; Zhang, Z.; Zhang, P.; Chen, K.; Plant Growth Regul. 83, 489-500 (2017)
No PubMed abstract available
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.9
ascorbate
required
Pohlia nutans
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
Pohlia nutans
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
construction of transgenic Arabidospis thaliana plants expressing the enzyme from Pohlia nutans, the Agrobacterium tumefaciens-mediated transformation of gene PnF3H confers tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, naringenin-feeding experiments and flavonoid metabolism profiling by HPLC analysis, phenotype, overview
Pohlia nutans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
required for activity
Pohlia nutans
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Pohlia nutans
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Pohlia nutans
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Pohlia nutans
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746071
Pohlia nutans
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746071
Pohlia nutans
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
F3H
-
Pohlia nutans
1.14.11.9
flavanone 3-hydroxylase
-
Pohlia nutans
1.14.11.9
PnF3H
-
Pohlia nutans
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.14.11.9
ascorbate
required
Pohlia nutans
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
gene PnF3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis and tree, quantitative real-time PCR enzyme expression analysis, functional recombinant expression in Arabidopsis thaliana ecotype Col-0 via Agrobacterium tumefaciens-mediated transformation conferring tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana
Pohlia nutans
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
construction of transgenic Arabidospis thaliana plants expressing the enzyme from Pohlia nutans, the Agrobacterium tumefaciens-mediated transformation of gene PnF3H confers tolerance to salt stress and abscisic acid treatment in transgenic Arabidopsis, naringenin-feeding experiments and flavonoid metabolism profiling by HPLC analysis, phenotype, overview
Pohlia nutans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.11.9
Fe2+
required for activity
Pohlia nutans
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Pohlia nutans
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Pohlia nutans
-
dihydrokaempferol + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746071
Pohlia nutans
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746071
Pohlia nutans
dihydrokaempferol + succinate + CO2
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.14.11.9
Pohlia nutans
PnF3H expression levels increase by 2.49fold at 1 h and then slightly decrease to 1.47fold at 24 h with cold treatment. For the simulated drought stress (i.e. 20% PEG 6000), the PnF3H expression levels enhance by 2.39fold at 3 h and the transcript abundance remains high up to 12 h. In the UV-B radiation, the PnF3H expression levels increase and reach the maximum level of 4.57fold at 3 h. After exogenous abscisic acid treatment, the PnF3H expression levels increase quickly and reach the maximum level of 2.57fold at 0.5 h
up
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
physiological function
the enzyme plays a role in plant salt stress resistance. PnF3H transcripts are induced by various stress and may play a positive role in stress tolerance
Pohlia nutans
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
physiological function
the enzyme plays a role in plant salt stress resistance. PnF3H transcripts are induced by various stress and may play a positive role in stress tolerance
Pohlia nutans
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.14.11.9
Pohlia nutans
PnF3H expression levels increase by 2.49fold at 1 h and then slightly decrease to 1.47fold at 24 h with cold treatment. For the simulated drought stress (i.e. 20% PEG 6000), the PnF3H expression levels enhance by 2.39fold at 3 h and the transcript abundance remains high up to 12 h. In the UV-B radiation, the PnF3H expression levels increase and reach the maximum level of 4.57fold at 3 h. After exogenous abscisic acid treatment, the PnF3H expression levels increase quickly and reach the maximum level of 2.57fold at 0.5 h
up