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Literature summary extracted from

  • Balusamy, S.; Rahimi, S.; Cho, Y.; Senthil, K.; Yang, D.
    Overexpression of geraniol 10-hydroxylase from Panax ginseng conferred enhanced resistance to Pseudomonas syringae in Arabidopsis (2017), Plant Growth Regul., 81, 305-316 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.83 gene G10H, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, quantitative RT-PCR enzyme expression analysis. Recombinant overexpression of the ginseng geraniol 10-hydroxylase P450 gene in Arabidopsis thaliana ecotype CS60000, from vector pCAMBIA1390 under control of promoter CaMV 35S, causes terpenoid indole alkaloid dihydrositsirikine production and also confers enhanced resistance to Pseudomonas syringae pv. tomato DC3000 Panax ginseng

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.83 membrane G10H is a transmembrane protein containing a transmembrane helix at the N-terminus Panax ginseng 16020
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2 Panax ginseng
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 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.83 Panax ginseng
-
-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.83 flower moderate enzyme expression level Panax ginseng
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1.14.14.83 leaf low enzyme expression level Panax ginseng
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1.14.14.83 additional information tissue-specific expression pattern and expression of PgG10H in response to various biotic and abiotic stresses Panax ginseng
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1.14.14.83 root high enzyme expression level Panax ginseng
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1.14.14.83 stem moderate enzyme expression level Panax ginseng
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.83 geraniol + [reduced NADPH-hemoprotein reductase] + O2
-
 Panax ginseng 10-hydroxygeraniol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.14.83 More secondary structure and three-dimensional structure analysis, overview Panax ginseng

Synonyms

EC Number Synonyms Comment Organism
1.14.14.83 G10H
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 Panax ginseng
1.14.14.83 geraniol 10-hydroxylase
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 Panax ginseng
1.14.14.83 PgCYP76C9
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 Panax ginseng
1.14.14.83 PgG10H
-
 Panax ginseng

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.83 cytochrome P450 a cytochrome P450 monooxygenase Panax ginseng
1.14.14.83 NADPH
-
 Panax ginseng

Expression

EC Number Organism Comment Expression
1.14.14.83 Panax ginseng tissue-specific expression pattern and expression of PgG10H in response to various biotic and abiotic stresses additional information
1.14.14.83 Panax ginseng the expression of PgG10H is differentially induced in ginseng, not only during Pseudomonas syringae infection and wounding but also after exposure to methyl jasmonate and salt stress up

General Information

EC Number General Information Comment Organism
1.14.14.83 metabolism the enzyme is involved in the secoiridoid pathway and the biosynthesis of terpenoid indole alkaloid via strictosidine Panax ginseng
1.14.14.83 additional information molecular docking is used for accurate prediction of protein-ligand interaction geometries at molecular level, molecular interaction studies, overview. Geraniol interacts with G10H residues Ala303 and Thr307 from the central helix I Panax ginseng