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Literature summary extracted from

  • Muangphrom, P.; Seki, H.; Suzuki, M.; Komori, A.; Nishiwaki, M.; Mikawa, R.; Fukushima, E.O.; Muranaka, T.
    Functional analysis of amorpha-4,11-diene synthase (ADS) homologs from non-artemisinin-producing artemisia species the discovery of novel koidzumiol and (+)-alpha-bisabolol synthases (2016), Plant Cell Physiol., 57, 1678-1688 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.114 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis Artemisia annua
1.14.14.114 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis Artemisia afra
1.14.14.114 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis Artemisia absinthium
4.2.3.24 expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Artemisia annua

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase] Artemisia annua
-
 artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase] Artemisia afra
-
 artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase] Artemisia absinthium
-
 artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase] Artemisia kurramensis
-
 artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
4.2.3.24 (2E,6E)-farnesyl diphosphate Artemisia annua
-
 amorpha-4,11-diene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.114 Artemisia absinthium K7ZPJ9
-
-
1.14.14.114 Artemisia afra K7ZR99
-
-
1.14.14.114 Artemisia annua Q1PS23
-
-
1.14.14.114 Artemisia kurramensis
-
-
-
4.2.3.24 Artemisia annua Q9AR04
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.114 leaf
-
 Artemisia annua
-
1.14.14.114 leaf
-
 Artemisia afra
-
1.14.14.114 leaf
-
 Artemisia absinthium
-
4.2.3.24 leaf
-
 Artemisia annua
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
-
 Artemisia annua artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
-
 Artemisia afra artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
-
 Artemisia absinthium artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.114 amorpha-4,11-diene + 3 O2 + [reduced NADPH-hemoprotein reductase]
-
 Artemisia kurramensis artemisinate + [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
4.2.3.24 (2E,6E)-farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.114 CYP71AV1
-
 Artemisia annua
1.14.14.114 CYP71AV1
-
 Artemisia afra
1.14.14.114 CYP71AV1
-
 Artemisia absinthium
1.14.14.114 CYP71AV1
-
 Artemisia kurramensis
4.2.3.24 ADS
-
 Artemisia annua

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.114 cytochrome P450
-
 Artemisia annua
1.14.14.114 cytochrome P450
-
 Artemisia afra
1.14.14.114 cytochrome P450
-
 Artemisia absinthium
1.14.14.114 cytochrome P450
-
 Artemisia kurramensis
1.14.14.114 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Artemisia annua
1.14.14.114 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Artemisia afra
1.14.14.114 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Artemisia absinthium
1.14.14.114 NADPH-hemoprotein reductase A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._ Artemisia kurramensis

General Information

EC Number General Information Comment Organism
1.14.14.114 metabolism the enzyme is involved in the artemisinin biosynthesis Artemisia annua
1.14.14.114 metabolism the enzyme is involved in the artemisinin biosynthesis Artemisia afra
1.14.14.114 metabolism the enzyme is involved in the artemisinin biosynthesis Artemisia absinthium
1.14.14.114 metabolism the enzyme is involved in the artemisinin biosynthesis Artemisia kurramensis
1.14.14.114 physiological function amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde Artemisia annua
1.14.14.114 physiological function amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde Artemisia afra
1.14.14.114 physiological function amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde Artemisia absinthium
1.14.14.114 physiological function amorpha-4,11-diene 12-monooxygenase (CYP71AV1) catalyzes the multistep oxidation of amorpha-4,11-diene to artemisinic alcohol and artemisinic aldehyde Artemisia kurramensis