Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, H.Y.; Babst, B.A.; Nyamdari, B.; Hu, H.; Sykes, R.; Davis, M.F.; Harding, S.A.; Tsai, C.J.
    Ectopic expression of a loblolly pine class II 4-coumarate CoA ligase alters soluble phenylpropanoid metabolism but not lignin biosynthesis in Populus (2014), Plant Cell Physiol., 55, 1669-1678 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli Rosetta 2 (DE3) cells Pinus taeda

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.01097
-
 4-coumarate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.0296
-
 caffeate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.0302
-
 4-coumarate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.0423
-
 caffeate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.0522
-
 ferulate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.0849
-
 ferulate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.8875
-
 cinnamate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 1.0803
-
 cinnamate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Pinus taeda preferred substrate AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Pinus taeda P41636 loblolly pine
-
6.2.1.12 Pinus taeda U5MY99 loblolly pine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12 His-Bind purification kit Pinus taeda

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.12 leaf
-
 Pinus taeda
-
6.2.1.12 additional information isoform 4CL3 is absent in xylem Pinus taeda
-
6.2.1.12 root highest expression in root tip Pinus taeda
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Pinus taeda AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA preferred substrate Pinus taeda AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Pinus taeda AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Pinus taeda AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA
-
 Pinus taeda AMP + diphosphate + feruloyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate:CoA ligase
-
 Pinus taeda
6.2.1.12 4CL1 isoform Pinus taeda
6.2.1.12 4CL3 isoform Pinus taeda

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 0.13
-
 caffeate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.15
-
 ferulate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.19
-
 4-coumarate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 0.27
-
 cinnamate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 1.6
-
 caffeate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 4.15
-
 4-coumarate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 5.22
-
 cinnamate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 14.99
-
 ferulate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Pinus taeda

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
6.2.1.12 0.3
-
 cinnamate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 1.8
-
 ferulate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 3.1
-
 caffeate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 4.8
-
 cinnamate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 17.3
-
 4-coumarate isoform 4CL3, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 54.1
-
 caffeate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 137.3
-
 4-coumarate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda
6.2.1.12 287.4
-
 ferulate isoform 4CL1, pH and temperature not specified in the publication Pinus taeda