EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.3.48 | gene Pltps3, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps3 emitted significantly more (S)-linalool and (3E)-4,8-dimethylnona-1,3,7-triene (DMNT) than wild-type plants, recombinant expression in Escherichia coli strain BL21(DE3) | Phaseolus lunatus |
4.2.3.48 | gene Pltps4, DNA and amino acid sequence determination and analysis, phylogenetic tree of terpene synthases (TPSs), recombinant expression in Oryza sativa ssp. japonica var. ZH11 plants using transformation method via Agrobacterium tumefaciens strain EHA105, real-time quantitative PCR enzyme expression analysis, transgenic rice expressing Pltps4 produces (S)-linalool, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) and (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), recombinant expression in Escherichia coli strain BL21(DE3) | Phaseolus lunatus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.48 | farnesyl diphosphate + H2O | Phaseolus lunatus | - |
(E)-nerolidol + diphosphate | - |
? | |
4.2.3.48 | geranyl diphosphate + H2O | Phaseolus lunatus | - |
(3S)-linalool + diphosphate | - |
? | |
4.2.3.48 | geranylgeranyl diphosphate + H2O | Phaseolus lunatus | - |
(6E,10E)-geranyllinalool + diphosphate | - |
? | |
4.2.3.48 | additional information | Phaseolus lunatus | enzyme PlTPS3 is able to convert GPP to linalool, and FPP to (E)-nerolidol, but PlTPS3 is unable to convert GGPP to (E,E)-geranyllinalool, no detection of TMTT | ? | - |
? | |
4.2.3.48 | additional information | Phaseolus lunatus | enzyme PlTPS4 is able to produce linalool, (E)-nerolidol, and (E,E)-geranyllinalool via GPP, FPP and GGPP, respectively | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.3.48 | Phaseolus lunatus | A0A1W5VLZ8 | cv. Sieva | - |
4.2.3.48 | Phaseolus lunatus | A0A1W5VM12 | cv. Sieva | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.3.48 | recombinant enzyme from Escherichia coli strain BL21(DE3) | Phaseolus lunatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.3.48 | farnesyl diphosphate + H2O | - |
Phaseolus lunatus | (E)-nerolidol + diphosphate | - |
? | |
4.2.3.48 | geranyl diphosphate + H2O | - |
Phaseolus lunatus | (3S)-linalool + diphosphate | - |
? | |
4.2.3.48 | geranylgeranyl diphosphate + H2O | - |
Phaseolus lunatus | (6E,10E)-geranyllinalool + diphosphate | - |
? | |
4.2.3.48 | additional information | no activity with geranylgeranyl diphosphate | Phaseolus lunatus | ? | - |
? | |
4.2.3.48 | additional information | enzyme PlTPS3 is able to convert GPP to linalool, and FPP to (E)-nerolidol, but PlTPS3 is unable to convert GGPP to (E,E)-geranyllinalool, no detection of TMTT | Phaseolus lunatus | ? | - |
? | |
4.2.3.48 | additional information | enzyme PlTPS4 is able to produce linalool, (E)-nerolidol, and (E,E)-geranyllinalool via GPP, FPP and GGPP, respectively | Phaseolus lunatus | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.3.48 | Pltps3 | - |
Phaseolus lunatus |
4.2.3.48 | Pltps4 | - |
Phaseolus lunatus |
4.2.3.48 | TPS3 | - |
Phaseolus lunatus |
4.2.3.48 | TPS4 | - |
Phaseolus lunatus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.2.3.48 | NADPH | - |
Phaseolus lunatus |
EC Number | Organism | Comment | Expression |
---|---|---|---|
4.2.3.48 | Phaseolus lunatus | treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus, induces the expression of gene Pltps3 | up |
4.2.3.48 | Phaseolus lunatus | treatment with either the elicitor alamethicin or spider mites, Tetranychus cinnabarinus, induces the expression of gene Pltps4 | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.3.48 | evolution | PlTP3 is, from a phylogenetic perspective, an ancestor monolineage member in the TPS-g subfamily | Phaseolus lunatus |
4.2.3.48 | physiological function | enzyme Pltps3 is involved in the biosynthesis of homoterpenes (also known as tetranorterpenes), i.e. (S)-linalool (EC 4.3.2.25) and the C11 homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT). PlTPS3 can discriminate GGPP from GPP and FPP, and is a specific terpene synthase, TPS, involved in DMNT biosynthesis in Phaseolus lunatus | Phaseolus lunatus |
4.2.3.48 | physiological function | enzyme Pltps3 is involved in the biosynthesis of homoterpenes (also known as tetranorterpenes), i.e. (S)-linalool, the C11 homoterpene (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), and the C16 homoterpene (E,E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene (TMTT), via production of the precursors linalool, (E)-nerolidol, and (E,E)-geranyllinalool from GPP, FPP and GGPP, respectively | Phaseolus lunatus |