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Literature summary extracted from

  • Hirata, K.; Poeaknapo, C.; Schmidt, J.; Zenk, M.H.
    1,2-Dehydroreticuline synthase, the branch point enzyme opening the morphinan biosynthetic pathway (2004), Phytochemistry, 65, 1039-1046 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.19.54 analysis [1-2H, 13C]-(R,S)-reticuline is enzymatically converted into [1-13C]-dehydroreticuline. Release of the hydrogen atom in position C-1 of the isoquinoline alkaloid during the oxidative conversion can be exploited as a sensitive assay system for the enzyme Papaver somniferum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.19.54 0.117
-
 (S)-reticuline pH 8.7, 35°C Papaver somniferum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.54 vesicle enzyme occurs in particles of different densities Papaver somniferum 31982
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.54 Papaver somniferum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.19.54 from seedling Papaver somniferum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.54 seedling
-
 Papaver somniferum
-

Storage Stability

EC Number Storage Stability Organism
1.14.19.54 enzyme is sensitive to freezing Papaver somniferum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.54 (S)-norreticuline + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum 1,2-dehydronorreticuline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.54 (S)-reticuline + [reduced NADPH-hemoprotein reductase] + O2
-
 Papaver somniferum 1,2-dehydroreticuline + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.19.54 additional information enzyme accepts both (S)-reticuline and (S)-norreticuline as substrates Papaver somniferum ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.19.54 37
-
-
 Papaver somniferum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.19.54 27
-
 50% of maximum activity Papaver somniferum
1.14.19.54 55
-
 50% of maximum activity Papaver somniferum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.19.54 8.8
-
-
 Papaver somniferum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.19.54 8
-
 50% of maximum activity Papaver somniferum
1.14.19.54 9.5
-
 50% of maximum activity Papaver somniferum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.54 additional information enzyme does not need a redox cofactor Papaver somniferum

General Information

EC Number General Information Comment Organism
1.14.19.54 physiological function enzyme provides the necessary inversion of configuration and completes the pathway from two molecules of L-tyrosine via (S)-norcoclaurine to (R)-reticuline in opium poppy involving a total number of 11 enzymes Papaver somniferum