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Literature summary extracted from

  • Liedschulte, V.; Schwaar, J.D.; Laparra, H.; Vuarnoz, A.; Philippon, B.; Bakaher, N.; Sierro, N.; Bovet, L.; Lang, G.; Goepfert, S.
    Identification of CYP82E21 as a functional nicotine N-demethylase in tobacco flowers (2016), Phytochemistry, 131, 9-16 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.B11 gene CYP82E21, unrooted phylogenetic analysis and tree of CYP82E21 and related CYP82E coding sequences, quantitative PCR enzyme expression analysis, ectopic expression of the coding sequence in a tobacco line lacking functional CYP82E4, CYP82E5 and CYP82E10 genes, resulting in an eightfold increase of nicotine demethylation compared to the control plants confirming the nicotine N-demethylase activity of CYP82E21 Nicotiana tabacum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.B11 additional information enzyme silencing by introducing a CYP82E21-specific RNAi construct in ovaries, silencing of CYP82E21 leads to up to 90% reduced nicotine conversion in flower ovaries Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.B11 nicotine + NADPH + H+ Nicotiana tabacum
-
 nornicotine + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.B11 Nicotiana tabacum
-
 var. TN90
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.B11 flower regarding sepals, petals, ovaries, stigma including style, filaments and anthers, CYP82E21 expression is found almost exclusively in ovaries, with very low expression levels observed in other flower organs Nicotiana tabacum
-
1.14.14.B11 additional information CYP82E21 expression pattern analysis, CYP82E21 is specifically expressed in ovaries but not expressed in leaves Nicotiana tabacum
-
1.14.14.B11 ovary specific for Nicotiana tabacum
-
1.14.14.B11 plant ovary specific for Nicotiana tabacum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.B11 nicotine + NADPH + H+
-
 Nicotiana tabacum nornicotine + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.B11 CYP82E21
-
 Nicotiana tabacum
1.14.14.B11 nicotine N-demethylase
-
 Nicotiana tabacum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.B11 cytochrome P450
-
 Nicotiana tabacum
1.14.14.B11 NADPH
-
 Nicotiana tabacum

General Information

EC Number General Information Comment Organism
1.14.14.B11 evolution N-demethylase enzymes (NND) belongs to the family of cytochrome P450 monooxygenases (CYP). The CYP82E21 gene is found in all Nicotiana tabacum cultivars analysed and originates from the tobacco ancestor Nicotiana tomentosiformis Nicotiana tabacum
1.14.14.B11 malfunction nornicotine formation can be reduced in ovaries by introducing a CYP82E21-specific RNAi construct Nicotiana tabacum
1.14.14.B11 physiological function the CYP82E21 gene encodes a functional ovary-specific nicotine N-demethylase, nornicotine is formed by oxidative demethylation of nicotine Nicotiana tabacum