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Literature summary extracted from

  • Koskela, M.M.; Dahlstroem, K.M.; Goni, G.; Lehtimaeki, N.; Nurmi, M.; Velazquez-Campoy, A.; Hanke, G.; Boelter, B.; Salminen, T.A.; Medina, M.; Mulo, P.
    Arabidopsis FNRL protein is an NADPH-dependent chloroplast oxidoreductase resembling bacterial ferredoxin-NADP+ reductases (2018), Physiol. Plant., 162, 177-190 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0016
-
 NADPH with 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.0 and 25°C Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 chloroplast
-
 Arabidopsis thaliana 9507
-

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Arabidopsis thaliana Q9XI55 ecotype Col-0
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 HisPur cobalt resin column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.18.1.2 leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 additional information the enzyme does not interact with Arabidopsis ferredoxin 1 Arabidopsis thaliana ?
-
 ?
1.18.1.2 oxidized 2,6-dichlorophenolindophenol + NADPH
-
 Arabidopsis thaliana reduced 2,6-dichlorophenolindophenol + NADP+ + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 ? x * 28000, SDS-PAGE Arabidopsis thaliana
1.18.1.2 ? x * 28800, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NADP+ oxidoreductase
-
 Arabidopsis thaliana
1.18.1.2 ferredoxin-NADP+ reductase
-
 Arabidopsis thaliana
1.18.1.2 FNR
-
 Arabidopsis thaliana
1.18.1.2 FNRL
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 3.2
-
 NADPH with 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.0 and 25°C Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD
-
 Arabidopsis thaliana
1.18.1.2 NADPH dependent on Arabidopsis thaliana

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 2000
-
 NADPH with 2,6-dichlorophenolindophenol as cosubstrate, at pH 7.0 and 25°C Arabidopsis thaliana