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Literature summary extracted from

  • Slabaugh, M.; Cooper, L.; Kishore, V.; Knapp, S.; Kling, J.
    Genes affecting novel seed constituents in Limnanthes alba Benth transcriptome analysis of developing embryos and a new genetic map of meadowfoam (2015), PeerJ, 2015, e915 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.10 gene DELTA5DS, gene mapping and genotyping, Construction and annotation of a developing embryo cDNA library, gene DELTA5DS DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR enzyme expression analysis Limnanthes alba subsp. alba

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.10 additional information the enzyme lacks a chloroplastic transit peptide Limnanthes alba subsp. alba
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.10 icosanoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ Limnanthes alba subsp. alba
-
 (Z)-icos-5-enoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.10 additional information Limnanthes alba subsp. alba very long chain fatty acids are desaturated at the DELTA5 position ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.10 Limnanthes alba subsp. alba
-
 cv. MF164, var. Ross
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.10 embryo the enzyme is highly expressed in mid-development embryos Limnanthes alba subsp. alba
-
1.14.19.10 seed
-
 Limnanthes alba subsp. alba
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.10 icosanoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
-
 Limnanthes alba subsp. alba (Z)-icos-5-enoyl-CoA + 2 ferricytochrome b5 + 2 H2O
-
 ?
1.14.19.10 additional information very long chain fatty acids are desaturated at the DELTA5 position Limnanthes alba subsp. alba ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.10 acyl-CoA DELTA5 desaturase
-
 Limnanthes alba subsp. alba
1.14.19.10 Delta-5 acyl-CoA desaturase
-
 Limnanthes alba subsp. alba
1.14.19.10 DELTA5 desaturase
-
 Limnanthes alba subsp. alba
1.14.19.10 DELTA5DS
-
 Limnanthes alba subsp. alba

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.10 cytochrome b5
-
 Limnanthes alba subsp. alba

General Information

EC Number General Information Comment Organism
1.14.19.10 evolution the enzyme is a member of the large DELTAa9-FADS-like or ADS subfamily found in eukaryotes including vertebrates, insects, higher plants and fungi, as well as a wide range of bacteria. These non-heme, iron-containing, endoplasmic reticulum membrane-bound enzymes exhibit a diversity of subcellular localization, lipid substrate utilization, regiospecificity, and biological function Limnanthes alba subsp. alba
1.14.19.10 metabolism mapping genes in meadowfoam glucosinolate metabolism, overview Limnanthes alba subsp. alba
1.14.19.10 additional information meadowfoam seed oil is the richest known source of DELTA5 monounsaturated fatty acids and presently the sole commercial source of DELTA5-unsaturated very long chain fatty acids, a high 20:1DELTA5 phenotype of meadowfoam oil Limnanthes alba subsp. alba