Literature summary extracted from

Guo, J.; Ma, X.; Cai, Y.; Ma, Y.; Zhan, Z.; Zhou, Y.J.; Liu, W.; Guan, M.; Yang, J.; Cui, G.; Kang, L.; Yang, L.; Shen, Y.; Tang, J.; Lin, H.; Ma, X.; Jin, B.; Liu, Z.; Peters, R.J.; Zhao, Z.K.; Huang, L.
Cytochrome P450 promiscuity leads to a bifurcating biosynthetic pathway for tanshinones (2016), New Phytol., 210, 525-534 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.14.14.67	expression in Saccharomyces cerevisiae	Salvia miltiorrhiza

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
1.14.14.67	homology modeling of structure	Salvia miltiorrhiza

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.14.14.60	Salvia miltiorrhiza	A0A0Y0GRS3	-	-
1.14.14.65	Salvia miltiorrhiza	A0A0Y0GRS3	-	-
1.14.14.67	Salvia miltiorrhiza	A0A125QZE2	-	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.14.14.60	root	expression is more abundant in the root than in aerial tissues	Salvia miltiorrhiza	-
1.14.14.65	root	expression is more abundant in the root than in aerial tissues	Salvia miltiorrhiza	-
1.14.14.67	hairy root	-	Salvia miltiorrhiza	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.14.14.60	ferruginol + [reduced NADPH-hemoprotein reductase] + O2	-	Salvia miltiorrhiza	11-hydroxyferruginol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
1.14.14.60	additional information	enzyme additionally oxidizes at C-7 to yield sugiol, reaction of EC 1.14.14.65	Salvia miltiorrhiza	?	-	?
1.14.14.60	sugiol + [reduced NADPH-hemoprotein reductase] + O2	-	Salvia miltiorrhiza	11-hydroxysugiol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
1.14.14.65	11-hydroxyferruginol + [reduced NADPH-hemoprotein reductase] + O2	-	Salvia miltiorrhiza	11-hydroxysugiol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
1.14.14.65	ferruginol + [reduced NADPH-hemoprotein reductase] + O2	-	Salvia miltiorrhiza	sugiol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
1.14.14.65	additional information	enzyme addtionally oxidizes ferruginol at C-11 to give 11-hydroxyferruginol, reaction of EC 1.14.14.60	Salvia miltiorrhiza	?	-	?
1.14.14.67	11-hydroxyferruginol + [reduced NADPH-hemoprotein reductase] + O2	-	Salvia miltiorrhiza	11,20-dihydroxyferruginol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
1.14.14.67	11-hydroxysugiol + [reduced NADPH-hemoprotein reductase] + O2	-	Salvia miltiorrhiza	11,20-dihydroxysugiol + [oxidized NADPH-hemoprotein reductase] + H2O	-	?
1.14.14.67	additional information	enzyme hydroxylates 11-hydroxyferruginol and 11-hydroxysugiol at C-20 to give 11,20-dihydroxyferruginal and 11,20-dihydroxysugiol	Salvia miltiorrhiza	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
1.14.14.60	CYP76AH3	-	Salvia miltiorrhiza
1.14.14.65	CYP76AH3	-	Salvia miltiorrhiza
1.14.14.67	CYP76AK1	-	Salvia miltiorrhiza

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.14.14.60	cytochrome P450	-	Salvia miltiorrhiza

General Information

EC Number	General Information	Comment	Organism
1.14.14.60	physiological function	cytochrome CYP76AH3 oxidizes ferruginol at two different carbon centers, and CYP76AK1 hydroxylates at C-20 of two of the resulting intermediates. Together, they convert ferruginol into 11,20-dihydroxy ferruginol and 11,20-dihydroxy sugiol en route to tanshinones. Silencing of CYP76AH3 leads to significant reduction in concentration of 11-hydroxyferruginol and 11-hydroxysugiol	Salvia miltiorrhiza

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Release 2023.1 (January 2023)