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Literature summary extracted from

  • Nieter, A.; Kelle, S.; Linke, D.; Berger, R.G.
    A p-coumaroyl esterase from Rhizoctonia solani with a pronounced chlorogenic acid esterase activity (2017), New Biotechnol., 37, 153-161 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.B10 heterologously expressed in Pichia pastoris. Recombinant enzyme production on the 5-L bioreactor scale exceeds the productivity of the wild type strain by a factor of 800 Rhizoctonia solani
3.1.1.42 expression in Pichia pastoris Rhizoctonia solani
3.1.1.73 expression in Pichia pastoris Rhizoctonia solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.B10 0.0183
-
 chlorogenic acid pH 6.0, 37°C Rhizoctonia solani
3.1.1.B10 0.0285
-
 methyl caffeate pH 6.0, 37°C Rhizoctonia solani
3.1.1.B10 0.0358
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.42 0.0183
-
 chlorogenate pH 6.0, 37°C Rhizoctonia solani
3.1.1.42 0.0358
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 0.0183
-
 chlorogenate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 0.0285
-
 methyl caffeate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 0.0358
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 0.0384
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 6.0, 37°C Rhizoctonia solani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.B10 extracellular
-
 Rhizoctonia solani
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.B10 Rhizoctonia solani A0A1D0BQV8
-
-
3.1.1.42 Rhizoctonia solani A0A1D0BQV8 cf. EC 3.1.1.73
-
3.1.1.73 Rhizoctonia solani A0A1D0BQV8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.B10
-
 Rhizoctonia solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.B10 additional information submerged cultures of Rhizoctonia solani grown in the presence of sugar beet pectin or Tween 80 Rhizoctonia solani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.B10 chlorogenic acid + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 ethyl cinnamate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 ethyl ferulate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 methyl caffeate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 methyl cinnamate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 methyl ferulate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 methyl p-coumarate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.B10 methyl sinapate + H2O
-
 Rhizoctonia solani ?
-
 ?
3.1.1.42 chlorogenate + H2O 83% of the activity with methyl p-coumarate Rhizoctonia solani caffeate + quinate
-
 ?
3.1.1.42 methyl p-coumarate + H2O
-
 Rhizoctonia solani methanol + p-coumarate
-
 ?
3.1.1.73 2-O-trans-feruloyl-alpha-L-arabinofuranosyl-(1->5)-L-arabinofuranose + H2O
-
 Rhizoctonia solani ferulic acid + alpha-L-arabinofuranosyl-(1->5)-L-arabinofuranose
-
 ?
3.1.1.73 5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Rhizoctonia solani ferulic acid + L-arabinofuranose
-
 ?
3.1.1.73 alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Rhizoctonia solani ferulic acid + alpha-L-galactopyranosyl-(1->2)-beta-D-xylopyranosyl-(1->2)-L-arabinofuranose
-
 ?
3.1.1.73 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Rhizoctonia solani ferulic acid + beta-D-xylopyranosyl-(1->2)-L-arabinofuranose
-
 ?
3.1.1.73 chlorogenate + H2O 83% of the activity with methyl p-coumarate Rhizoctonia solani caffeate + quinate
-
 ?
3.1.1.73 coffee pulp + H2O
-
 Rhizoctonia solani caffeate + ferulate + p-coumarate + ?
-
 ?
3.1.1.73 D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-5-O-trans-feruloyl-L-arabinofuranose + H2O
-
 Rhizoctonia solani ferulic acid + D-xylopyranosyl-(1->4)-beta-D-xylopyranosyl-(1->3)-L-arabinofuranose
-
 ?
3.1.1.73 destarched wheat bran + H2O
-
 Rhizoctonia solani caffeate + ferulate + p-coumarate + ?
-
 ?
3.1.1.73 ethyl ferulate + H2O
-
 Rhizoctonia solani ethanol + ferulate
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Rhizoctonia solani methanol + caffeate
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Rhizoctonia solani methanol + ferulate
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Rhizoctonia solani methanol + p-coumarate
-
 ?
3.1.1.73 sugar beet pectin + H2O
-
 Rhizoctonia solani caffeate + ferulate + p-coumarate + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.B10 FAE3
-
 Rhizoctonia solani
3.1.1.42 FAE3
-
 Rhizoctonia solani
3.1.1.73 FAE3
-
 Rhizoctonia solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.B10 30
-
-
 Rhizoctonia solani
3.1.1.42 30
-
-
 Rhizoctonia solani
3.1.1.73 30
-
-
 Rhizoctonia solani

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.B10 20 40 20°C: about 75% of maximal activity, 40°C: about 60% of maximal activity. The enzyme still possesses more than 80% residual activity at 37°C, activity decreases drastically above 40°C, reaching a plateau of approximately 10% activity between 50°C and 90°C Rhizoctonia solani
3.1.1.42 37
-
 more than 80% of maximum activity Rhizoctonia solani
3.1.1.42 40
-
 rapid loss of activity above Rhizoctonia solani
3.1.1.73 37
-
 more than 80% of maximum activity Rhizoctonia solani
3.1.1.73 40
-
 rapid loss of activity above Rhizoctonia solani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.B10 30
-
 1 h, 55% residual activity Rhizoctonia solani
3.1.1.B10 40
-
 1 h, activity is almost lost Rhizoctonia solani
3.1.1.42 30
-
 1 h, 55% residual activity Rhizoctonia solani
3.1.1.42 40
-
 1 h, almost complete loss of activity Rhizoctonia solani
3.1.1.73 30
-
 1 h, 55% residual activity Rhizoctonia solani
3.1.1.73 40
-
 1 h, almost complete loss of activity Rhizoctonia solani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.B10 20
-
 methyl caffeate pH 6.0, 37°C Rhizoctonia solani
3.1.1.B10 29.3
-
 chlorogenic acid pH 6.0, 37°C Rhizoctonia solani
3.1.1.B10 33.1
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.42 29.3
-
 chlorogenate pH 6.0, 37°C Rhizoctonia solani
3.1.1.42 33.1
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 20
-
 methyl caffeate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 29.1
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 29.3
-
 chlorogenate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 33.1
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.B10 6
-
 substrate: methyl ferulate Rhizoctonia solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.B10 4 7 the enzyme retaines more than 50% residual activity in the pH range 4.0-7.0. At pH 7.5 less than 10% residual activity is detected, while no activity is found at pH 8.0, substrate: methyl ferulate Rhizoctonia solani

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.B10 701.8
-
 methyl caffeate pH 6.0, 37°C Rhizoctonia solani
3.1.1.B10 925.3
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.B10 1598.4
-
 chlorogenic acid pH 6.0, 37°C Rhizoctonia solani
3.1.1.42 925
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.42 1598
-
 chlorogenate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 702
-
 methyl caffeate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 759
-
 beta-D-xylopyranosyl-(1->2)-5-O-trans-feruloyl-L-arabinofuranose pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 925
-
 methyl p-coumarate pH 6.0, 37°C Rhizoctonia solani
3.1.1.73 1598
-
 chlorogenate pH 6.0, 37°C Rhizoctonia solani