Literature summary extracted from

Chatterjee, A.; Abeydeera, N.; Bale, S.; Pai, P.; Dorrestein, P.; Russell, D.; Ealick, S.; Begley, T.
Saccharomyces cerevisiae THI4p is a suicide thiamine thiazole synthase (2011), Nature, 478, 542-546 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.2.60	expression in Escherichia coli	Saccharomyces cerevisiae

Protein Variants

EC Number	Protein Variants	Comment	Organism
2.4.2.60	C204A	partially active mutant	Saccharomyces cerevisiae
2.4.2.60	H200N	partially active mutant	Saccharomyces cerevisiae
2.4.2.60	R301Q	inactive	Saccharomyces cerevisiae

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.4.2.60	Iron	iron-dependent sulfide transfer reaction from the protein to a reaction intermediate	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.2.60	additional information	Saccharomyces cerevisiae	Thi4 is a suicidal enzyme undergoing only a single turnover	?	-	?
2.4.2.60	NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine	Saccharomyces cerevisiae	-	nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.2.60	Saccharomyces cerevisiae	P32318	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.2.60	additional information	Thi4 is a suicidal enzyme undergoing only a single turnover	Saccharomyces cerevisiae	?	-	?
2.4.2.60	NAD+ + glycine + [ADP-thiazole synthase]-L-cysteine	-	Saccharomyces cerevisiae	nicotinamide + ADP-5-ethyl-4-methylthiazole-2-carboxylate + [ADP-thiazole synthase]-dehydroalanine + 3 H2O	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.4.2.60	Thi4	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)