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Literature summary extracted from

  • Liu, X.; Zhao, B.; Sun, L.; Bhuripanyo, K.; Wang, Y.; Bi, Y.; Davuluri, R.; Duong, D.; Nanavati, D.; Yin, J.; Kiyokawa, H.
    Orthogonal ubiquitin transfer identifies ubiquitination substrates under differential control by the two ubiquitin activating enzymes (2017), Nat. Commun., 8, 14286 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.45 gene Uba1, recombinant expression of His-tagged wild-type and mutant enzmyes from in Escherichia coli strain BL21, recombinant coexpression FLAG-tagged pairs of an engineered ubiquitin and engineered Uba1 or Uba6, that are generated for exclusive interactions, in HEK-293 cells from lentiviral vectors, 697 potential Uba6 targets and 527 potential Uba1 targets with 258 overlaps are identified Homo sapiens
6.2.1.45 gene Uba6, recombinant expression of His-tagged wild-type and mutant enzmyes from in Escherichia coli strain BL21, recombinant coexpression FLAG-tagged pairs of an engineered ubiquitin and engineered Uba1 or Uba6, that are generated for exclusive interactions, in HEK-293 cells from lentiviral vectors, 697 potential Uba6 targets and 527 potential Uba1 targets with 258 overlaps are identified Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.45 D616R site-directed mutagenesis Homo sapiens
6.2.1.45 D623R site-directed mutagenesis Homo sapiens
6.2.1.45 E601R site-directed mutagenesis Homo sapiens
6.2.1.45 H614R site-directed mutagenesis Homo sapiens
6.2.1.45 additional information enzyme silencing in HEK293 cells by lentiviral expression of shRNA. Generation of orthogonal pairs of xUB-xUBA6 and xUB-xUBA1, analysis of orthogonal interaction of xUB-xE1 in mammalian cells and identification of xUB-conjugated proteins. 697 potential Uba6 targets and 527 potential Uba1 targets with 258 overlaps are identified Homo sapiens
6.2.1.45 Q608R site-directed mutagenesis Homo sapiens
6.2.1.45 S621R site-directed mutagenesis Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.45 Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine Homo sapiens
-
 AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?
6.2.1.45 additional information Homo sapiens orthogonal ubiquitin transfer (OUT) technology to profile their ubiquitination targets in mammalian cells of isozymes Uba1 and Uba6 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.45 Homo sapiens A0AVT1
-
-
6.2.1.45 Homo sapiens P22314
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.45 recombinant FLAG-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21 by affinity chromatography Homo sapiens
6.2.1.45 recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21 by nickel affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.45 epithelial cell
-
 Homo sapiens
-
6.2.1.45 mammary gland
-
 Homo sapiens
-
6.2.1.45 MCF-10A cell
-
 Homo sapiens
-
6.2.1.45 PC-3M cell
-
 Homo sapiens
-
6.2.1.45 prostate
-
 Homo sapiens
-
6.2.1.45 prostate cancer cell
-
 Homo sapiens
-
6.2.1.45 RWPE-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.45 ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine
-
 Homo sapiens AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine
-
 ?
6.2.1.45 additional information orthogonal ubiquitin transfer (OUT) technology to profile their ubiquitination targets in mammalian cells of isozymes Uba1 and Uba6 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.45 Uba1
-
 Homo sapiens
6.2.1.45 Uba6
-
 Homo sapiens
6.2.1.45 ubiquitin activating enzyme E1
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.45 ATP
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
6.2.1.45 physiological function protein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquitin conjugating enzyme E2 and ubiquitin ligase E3. Ezrin and CUGBP1 undergo Uba6-dependent polyubiquitination. Uba1 is not required, in contrast to Uba6, for polyubiquitination and proteasomal degradation of ezrin and CUGBP1. Distinctive substrate pools exist for Uba1 and Uba6 that reflect non-redundant biological roles for Uba6 Homo sapiens
6.2.1.45 physiological function protein ubiquitination is mediated sequentially by ubiquitin activating enzyme E1, ubiquitin conjugating enzyme E2 and ubiquitin ligase E3. Polyubiquitination and proteasomal degradation of ezrin and CUGBP1 require Uba6, but not Uba1. Ezrin and CUGBP1 undergo Uba6-dependent polyubiquitination. Uba6 is involved in the control of ezrin localization and epithelial morphogenesis. Distinctive substrate pools exist for Uba1 and Uba6 that reflect non-redundant biological roles for Uba6 Homo sapiens