Literature summary extracted from
Fan, M.; Xiao, Y.; Li, M.; Chang, W.
Crystal structures of Arabidopsis thaliana oxalyl-CoA synthetase essential for oxalate degradation (2016), Mol. Plant, 9, 1349-1352 .
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
6.2.1.8 |
apo, ATP-bound, as well as oxalate- and AMP-bound enzyme states, hanging drop vapor diffusion method, using |
Arabidopsis thaliana |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
6.2.1.8 |
H214A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
Arabidopsis thaliana |
6.2.1.8 |
H319A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
Arabidopsis thaliana |
6.2.1.8 |
K500A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
Arabidopsis thaliana |
6.2.1.8 |
R409A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
Arabidopsis thaliana |
6.2.1.8 |
S289A |
the mutant shows strongly reduced activity compared to the wild type enzyme |
Arabidopsis thaliana |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
6.2.1.8 |
0.04215 |
- |
oxalate |
pH and temperature not specified in the publication |
Arabidopsis thaliana |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
6.2.1.8 |
Mg2+ |
required in form of Mg-ATP |
Arabidopsis thaliana |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
6.2.1.8 |
ATP + oxalate + CoA |
Arabidopsis thaliana |
oxalate is the specific substrate |
AMP + diphosphate + oxalyl-CoA |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.2.1.8 |
Arabidopsis thaliana |
Q9SMT7 |
- |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
6.2.1.8 |
ATP + oxalate + CoA |
oxalate is the specific substrate |
Arabidopsis thaliana |
AMP + diphosphate + oxalyl-CoA |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.2.1.8 |
AAE3 |
acyl-activating enzyme3, gene name |
Arabidopsis thaliana |
6.2.1.8 |
Oxalyl-CoA synthetase |
- |
Arabidopsis thaliana |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
6.2.1.8 |
ATP |
- |
Arabidopsis thaliana |
|
General Information
EC Number |
General Information |
Comment |
Organism |
---|
6.2.1.8 |
physiological function |
enzyme-dependent degradation of oxalate is important for normal seed development and for defense against oxalate-producing fungal pathogens |
Arabidopsis thaliana |