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Literature summary extracted from

  • Fan, M.; Xiao, Y.; Li, M.; Chang, W.
    Crystal structures of Arabidopsis thaliana oxalyl-CoA synthetase essential for oxalate degradation (2016), Mol. Plant, 9, 1349-1352 .
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.2.1.8 apo, ATP-bound, as well as oxalate- and AMP-bound enzyme states, hanging drop vapor diffusion method, using Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.8 H214A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
6.2.1.8 H319A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
6.2.1.8 K500A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
6.2.1.8 R409A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana
6.2.1.8 S289A the mutant shows strongly reduced activity compared to the wild type enzyme Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.8 0.04215
-
oxalate pH and temperature not specified in the publication Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.8 Mg2+ required in form of Mg-ATP Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA Arabidopsis thaliana oxalate is the specific substrate AMP + diphosphate + oxalyl-CoA
-
?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.8 Arabidopsis thaliana Q9SMT7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.8 ATP + oxalate + CoA oxalate is the specific substrate Arabidopsis thaliana AMP + diphosphate + oxalyl-CoA
-
?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.8 AAE3 acyl-activating enzyme3, gene name Arabidopsis thaliana
6.2.1.8 Oxalyl-CoA synthetase
-
Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.8 ATP
-
Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
6.2.1.8 physiological function enzyme-dependent degradation of oxalate is important for normal seed development and for defense against oxalate-producing fungal pathogens Arabidopsis thaliana