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Literature summary extracted from

  • Qiu, Z.; Tan, H.; Zhou, S.; Cao, L.
    Surface display of a bifunctional glutathione synthetase on Saccharomyces cerevisiae for converting chicken feather hydrolysate into glutathione (2014), Mol. Biotechnol., 56, 726-730 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.2 gene gcsgs, recombinant functional enzyme expression on the cell surface of Saccharomyces cerevisiae strain EBY100, for cell surface expression, the gcsgs gene is fused with the 3'-end region of the alpha-agglutinin gene and cloned into plasmid YD1-GCSGS, recombinant expression in and secretion from Escherichia coli strain DH5alpha Streptococcus thermophilus
6.3.2.3 gene gcsgs, recombinant functional enzyme expression on the cell surface of Saccharomyces cerevisiae strain EBY100, for cell surface expression, the gcsgs gene is fused with the 3'-end region of the alpha-agglutinin gene and cloned into plasmid YD1-GCSGS, recombinant expression in and secretion from Escherichia coli strain DH5alpha Streptococcus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.2 additional information to convert feather hydrolysates into GSH with high values, the bifunctional glutathione synthetase, that comprises the activities of EC 6.3.2.2 and EC 6.3.2.3, encoded by gcsgs from Streptococcus thermophilus is surface displayed on Saccharomyces cerevisiae strain EBY100, the potential in glutathione (GSH) production from feather hydrolysates is analyzed. The surface-displayed GCSGS can be used to convert feather hydrolysates into GSH. 10 g/l of feather are converted into 321.8 mg/l GSH by the Trichoderma atroviride F6, with feather degrading ability, and surface-displayed GCSGS. Method optimization, overview Streptococcus thermophilus
6.3.2.3 additional information to convert feather hydrolysates into GSH with high values, the bifunctional glutathione synthetase, that comprises the activities of EC 6.3.2.2 and EC 6.3.2.3, encoded by gcsgs from Streptococcus thermophilus is surface displayed on Saccharomyces cerevisiae strain EBY100, the potential in glutathione (GSH) production from feather hydrolysates is analyzed. The surface-displayed GCSGS can be used to convert feather hydrolysates into GSH. 10 g/l of feather are converted into 321.8 mg/l GSH by the Trichoderma atroviride F6, with feather degrading ability, and surface-displayed GCSGS. Method optimization, overview Streptococcus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.2 Mg2+ required Streptococcus thermophilus
6.3.2.3 Mg2+ required Streptococcus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine Streptococcus thermophilus
-
 ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine Streptococcus thermophilus
-
 ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.2 Streptococcus thermophilus D7P1H2
-
-
6.3.2.3 Streptococcus thermophilus D7P1H2
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.2 ATP + L-glutamate + L-cysteine
-
 Streptococcus thermophilus ADP + phosphate + gamma-L-glutamyl-L-cysteine
-
 ?
6.3.2.3 ATP + gamma-L-glutamyl-L-cysteine + glycine
-
 Streptococcus thermophilus ADP + phosphate + glutathione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.2 bifunctional glutathione synthetase
-
 Streptococcus thermophilus
6.3.2.2 GCSGS
-
 Streptococcus thermophilus
6.3.2.3 bifunctional glutathione synthetase
-
 Streptococcus thermophilus
6.3.2.3 GCSGS
-
 Streptococcus thermophilus
6.3.2.3 GSH synthetase
-
 Streptococcus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.2 30
-
 recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae Streptococcus thermophilus
6.3.2.3 30
-
 recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae Streptococcus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.2 8
-
 recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae Streptococcus thermophilus
6.3.2.3 8
-
 recombinant cell surface-displayed enzyme in Saccharomyces cerevisiae Streptococcus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.2 ATP
-
 Streptococcus thermophilus
6.3.2.3 ATP
-
 Streptococcus thermophilus