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Literature summary extracted from

  • Zhao, W.; Yang, J.; Tian, Y.; Fu, X.; Zhu, B.; Xue, Y.; Gao, J.; Han, H.J.; Peng, R.; Yao, Q.H.
    Expression, purification, and characterization of recombinant mangrove glutamine synthetase (2014), Mol. Biol. Rep., 41, 7575-7583 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of His-tagged enzyme in Escherichia coli strain glnA mutant JW3841-1, the recombinant enzyme can complement the auxotrophic strain Avicennia marina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.1.2 cytosol
-
 Avicennia marina 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Al3+ results in 8.91tivity compared to Mg2+ Avicennia marina
6.3.1.2 Ca2+ results in 43.9% activity compared to Mg2+ Avicennia marina
6.3.1.2 Co2+ results in 109.1% activity compared to Mg2+ Avicennia marina
6.3.1.2 Cu2+ results in 22.2% activity compared to Mg2+ Avicennia marina
6.3.1.2 Fe2+ results in 22.3% activity compared to Mg2+ Avicennia marina
6.3.1.2 Mg2+ required Avicennia marina
6.3.1.2 Mn2+ results in 17.12% activity compared to Mg2+ Avicennia marina
6.3.1.2 Ni2+ results in 8.92% activity compared to Mg2+ Avicennia marina
6.3.1.2 Zn2+ results in 9.58% activity compared to Mg2+ Avicennia marina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3 Avicennia marina
-
 ADP + phosphate + L-glutamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Avicennia marina Q9AXD8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 recombinant His-tagged enzyme from Escherichia coli strain glnA mutant JW3841-1 by nickel affinity chromatography and dialysis Avicennia marina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + L-glutamate + NH3
-
 Avicennia marina ADP + phosphate + L-glutamine
-
 r

Subunits

EC Number Subunits Comment Organism
6.3.1.2 ? x * 39300, recombinant His-tagged enzyme, SDS-PAGE Avicennia marina

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 AmGLN1
-
 Avicennia marina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 35
-
 assay at Avicennia marina

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.3.1.2 30 42 activity range Avicennia marina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 45
-
 purified recombinant enzyme, enzymatic activity of AmGLN1 is lost after 30 min of incubation at 45°C Avicennia marina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 6.5
-
 rcombinant enzyme Avicennia marina

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
6.3.1.2 additional information
-
 the remarkable stability of the enzyme under alkaline pH might be an environmental adaptation of Avicennia marina that grows in a highly alkaline environment Avicennia marina
6.3.1.2 4 10 the enzyme is fairly stable at pH 4.0-10.0, while he highest stability is observed at pH 7.0-9.0 Avicennia marina

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.2 ATP
-
 Avicennia marina

General Information

EC Number General Information Comment Organism
6.3.1.2 metabolism the glutamine synthetase/glutamate synthase cycle is considered as the major pathway for ammonium assimilation and regulation of nitrogen metabolism in higher plants Avicennia marina
6.3.1.2 physiological function glutamine synthetase catalyzes the formation of glutamine from glutamate in the presence of NH4+, ATP, and metal cations. The reaction is the rate-limiting step in the control of N-assimilation and N-recycling during growth and development of plants Avicennia marina