BRENDA - Enzyme Database

DELTA9 desaturase from Trypanosoma cruzi key enzyme in the parasite metabolism. Cloning and overexpression

Woelke, M.R.; Paulucci, N.S.; Selva, A.; Garban, H.; de Lema, M.G.; Microbiol. Res. 194, 29-37 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.28
DNA and amino acid sequence determination and analysis, functional overexpression of His6-tagged enzyme in Escherichia coli strain BL21 codon Plus, subcloning in Escherichia coli strain XL 10 GOLD, TcDELTA9DES-expressing Escherichia coli cells exhibit high desaturase activity associated with increased levels of monounsaturated fatty acids. The fatty acid composition of Escherichia coli shows that the recombinant desaturase can use 16:0 as substrate
Trypanosoma cruzi
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.19.28
ketoconazole
-
Trypanosoma cruzi
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.28
membrane
membrane-bound
Trypanosoma cruzi
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Trypanosoma cruzi
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Trypanosoma cruzi Tulahuen
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.28
Trypanosoma cruzi
G8FL60
-
-
1.14.19.28
Trypanosoma cruzi Tulahuen
G8FL60
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.19.28
native enzyme from epimastigotes partially by ultracentrifugation, recombinant His6-tagged enzyme from Escherichia coli strain BL21 codon Plus cell-free extract by nickel affinity chromatography
Trypanosoma cruzi
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.28
epimastigote
-
Trypanosoma cruzi
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745715
Trypanosoma cruzi
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745715
Trypanosoma cruzi Tulahuen
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 50000, about, recombinant enzyme, SDS-PAGE
Trypanosoma cruzi
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.19.28
37
-
in vivo assay at
Trypanosoma cruzi
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.28
Ferredoxin
-
Trypanosoma cruzi
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.19.28
Trypanosoma cruzi
about, isoelectric focusing
-
9
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.28
DNA and amino acid sequence determination and analysis, functional overexpression of His6-tagged enzyme in Escherichia coli strain BL21 codon Plus, subcloning in Escherichia coli strain XL 10 GOLD, TcDELTA9DES-expressing Escherichia coli cells exhibit high desaturase activity associated with increased levels of monounsaturated fatty acids. The fatty acid composition of Escherichia coli shows that the recombinant desaturase can use 16:0 as substrate
Trypanosoma cruzi
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.28
Ferredoxin
-
Trypanosoma cruzi
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.19.28
ketoconazole
-
Trypanosoma cruzi
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.28
membrane
membrane-bound
Trypanosoma cruzi
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Trypanosoma cruzi
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Trypanosoma cruzi Tulahuen
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.28
native enzyme from epimastigotes partially by ultracentrifugation, recombinant His6-tagged enzyme from Escherichia coli strain BL21 codon Plus cell-free extract by nickel affinity chromatography
Trypanosoma cruzi
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.19.28
epimastigote
-
Trypanosoma cruzi
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745715
Trypanosoma cruzi
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745715
Trypanosoma cruzi Tulahuen
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 50000, about, recombinant enzyme, SDS-PAGE
Trypanosoma cruzi
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.19.28
37
-
in vivo assay at
Trypanosoma cruzi
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.14.19.28
Trypanosoma cruzi
about, isoelectric focusing
-
9
General Information
EC Number
General Information
Commentary
Organism
1.14.19.28
metabolism
desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. This DELTA9-desaturation reaction normally requires molecular oxygen and reducing equivalents derived from NAD(P)H through an electron system consisting of either NADPH, cytochrome b5 and cytochrome b5-reductase in animals and fungi or ferredoxin-NADP+ oxidoreductase and ferredoxin in bacteria and plants
Trypanosoma cruzi
1.14.19.28
physiological function
the enzyme plays a critical role in the metabolism of the parasite
Trypanosoma cruzi
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.28
metabolism
desaturases, key enzymes in the metabolism of fatty acids, regulate the physical and biochemical properties of membranes. They adjust the composition of saturated and unsaturated fatty acids in response to changes in the environmental. This DELTA9-desaturation reaction normally requires molecular oxygen and reducing equivalents derived from NAD(P)H through an electron system consisting of either NADPH, cytochrome b5 and cytochrome b5-reductase in animals and fungi or ferredoxin-NADP+ oxidoreductase and ferredoxin in bacteria and plants
Trypanosoma cruzi
1.14.19.28
physiological function
the enzyme plays a critical role in the metabolism of the parasite
Trypanosoma cruzi